Mutagenic Variability in M2 Generation of Clusterbean [Cyamopsis tetragonoloba (L.) Taub.]
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Date
2022
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Abstract
The present investigation was carried out in a set of 322 M2
mutant lines along with 5 check varieties of clusterbean. The progenies
employed in the present investigation were derived from parents RGC
1038 and RGr 12-1 treated with three doses of sodium azide (0.3 mM,
0.4 mM and 0.5 mM), three doses of gamma rays (300 Gy, 400 Gy and
500 Gy) and their combination (400 Gy with 0.3 mM, 0.4 mM and 0.5
mM) in seven blocks in Augmented Randomized Block Design during
kharif, 2021. 161 progenies of each variety were evaluated for macro and
micro mutational changes. In the progenies of RGC 1038, higher
frequency of morphological mutants was detected as compared to RGr
12-1. Among chlorophyll deficient mutants, only xantha types were
observed in both the varieties with higher frequency in RGr 12-1 as
compared to RGC 1038.
The analysis of variance showed significant differences among
the mutant lines for all of the traits studied, viz., days to 50 per cent
flowering, days to maturity, plant height, branches per plant, clusters per
plant, pods per cluster, pods per plant, pod length, seeds per pod, 1000-
seed weight, seed yield per plant and chlorophyll content, indicated the
existence of significant variability among the mutants.
The high PCV and GCV were observed for seed yield per plant,
pods per cluster and pods per plant. All the characters exhibited high
heritability except chlorophyll content. Most of the characters showed
high genetic advance as per cent of mean. Test weight and chlorophyll content showed moderate genetic gain while days to 50 per cent
flowering and days to maturity revealed low genetic advance as per cent
of mean. High genetic advance as per cent of mean coupled with high
heritability was observed for plant height, branches per plant, clusters per
plant, pods per cluster, pods per plant, pod length, seeds per pod and
seed yield per plant. Selection based on these characters will be
rewarding for the improvement of clusterbean.
The character association analysis revealed that plant height,
clusters per plant, pods per cluster, pods per plant, pod length, seeds per
pod, test weight and days to maturity had positive and significant
correlation with seed yield per plant.
The path analysis reported that seeds per pod, pods per plant,
test weight and pods per cluster were effective for selecting high yielding
mutants as they exhibited high positive direct effect along with a
significant positive correlation with seed yield per plant.
Based on seed yield per plant, ten high yielding mutant lines
viz., T4-66, T2-31, T1-15, T4-57, T5-88, T9-151 identified from the mutant
progenies of RGC 1038 and T4-217, T8-304, T6-256 and T2-193 from
the progenies of RGr 12-1 could be used in clusterbean breeding
programme for generating elite mutant cultivars or through
hybridization for obtaining desirable segregants in the subsequent
generations.