MOLECULAR CHARACTERIZATION OF GENETIC MALE STERILE GENOTYPES IN COTTON (Gossypium Sp.)
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Date
2004
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UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BANGALORE
Abstract
"Cotton is the most important textile fibre and knowledge on
genetic relatedness among the advanced lines is essential for crop
improvement. The genetic diversity analysis of GMS genotypes
through molecular markers will be useful in diversifying the genotypes
for creation of hybrid combination. Application of molecular markers
is an interesting alternative for selecting male sterile plants. Twenty
near isogenic diploid GMS lines were screened using 119 random
decamer primers of which 82 markers were found to polymorphic with
61.7 per cent polymorphism. Out of 314 amplicons amplified, 187
were found to be polymorphic, with an average of 3.83 fragments per
primer of which 2.28 were polymorphic. The similarity among
genotypes ranged from 70 to 98 per cent. Genetic diversity analysis
among fourteen near isogenic tetraploid GMS lines was carried out
using 88 random decamer primers. Out of 310 fragments amplified,
190 were found to be polymorphic with an average of 5.44 fragments
per primer, 3.33 fragments per primer were polymorphic. OPY primers
were found to be highly polymorphic. Presence of genetic diversity was
evidenced by genetic similarity indices (0.76 - 0.98). The sterile and
fertile plants of different genotypes made independent clusters
indicating their divergence.
RAPD markers are used as a tool for estimating genetic diversity
and can be used on continuing basis to document the available
variability in the cotton germplasm. Since G. hirsutum and G.arboreum
groups have been improved independently, these form separate
clusters, depicting enormous variation among them despite having
same genome. Markers OPB04 and 0PZ14 showed genetic diversity
between fertile and sterile plants of all the diploid GMS genotypes.
Similarly the marker OPB04 also showed genetic diversity within
fertile and sterile plants of the tetraploid GMS genotypes. Hence, they
can be considered as putative markers for linkage studies and
identification of male sterile and fertile plants."
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