Effect of herbal and vermi additives on growth, survival and disease resistance in gold fish, Carassius auratus auratus
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Date
2015
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Publisher
CCSHAU
Abstract
Ornamental fish culture is fast emerging as a major branch of aquaculture globally. Aquarium keeping
is the second largest hobby in the world next to photography. The ornamental fish and aquatic plant
industry is gaining importance due to its tremendous economic opportunities and prospects. The world
ornamental fish trade is to the tune of US$6 billion and they are easily susceptible to bacterial diseases.
So to deal with such problems diseased gold fish Crassius auratus auratus were collected from the
local market on the basis of their physical appearance and its movement in aquarium. Diseased tissue
was taken, homogenised and bacteria were isolated on nutrient agar by spread plate method. Bacterial
isolates were selected on the basis of colony morphology. In total two bacterial isolates were selected
and purified by streaking on nutrient agar plate. Gram staining of bacterial isolates was performed. For
identification of isolates, genomic DNA was extracted using CTAB method. After sequencing it was
clear that two bacteria were Aeromonas hydrophila and Pseudomonas fluorescence out of which
Aeromonas. hydrophila is more pathogenic, after incubating bacteria in the gold fish. When four
different herbs and three different species of earthworm extract were used against bacteria isolated
from gold fish. Out of four herbal extract Amla and out of three Earthworms species Eisenia fetida
extract has maximum zone of inhibition against bacteria and there is significant increase in Hb, RBC,
PCV, TLC, Length and Weight of gold fish after 90 days of incubation@ 2%/kg feed. These
parameters were more for Amla extract as compared to Eisenia fetida extract. Both the extract also
help in improving fish health after infestation with A. hydrophila which is clear from the histological
slides of liver and intestine.
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Keywords
Bacteria, Biological phenomena, Diseases, Extraction, Pathogens, Animal husbandry, Application methods, Biological development, Irrigation, Antibiotics