Association Analysis of Charcoal Rot Disease and Yield Component Traits in Sorghum Minicore Germplasm with EST-SSR and Allelic Variation in SbRGA114
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Date
2016-03
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University of Agricultural Science, Dharwad
Abstract
Evaluation of sorghum minicore consisting of 242 accessions during rabi season (2014-15) at RARS, Vijayapura revealed significant variations both at phenotypic and genotypic level for charcoal rot disease and yield component traits. High values of PCV, GCV and heritability coupled with GA was recorded for percent lodging, plant height, panicle exertion, earhead length, earhead width, number of leaves per plant, 100 seed weight, seed yield per plant.
SbRGA114 based association for charcoal rot component traits in a set of 30 sorghum minicore accessions revealed nucleotide changes at seven positions with minor allele frequency(MAF) of >5%. Six significant associations (P<0.05) were observed for nucleotide changes at five positions (SbRGA114 86, SbRGA114 102, SbRGA114 944, SbRGA114 1278, SbRGA114 110) with phenotypic variation ranging from 2.9- 7.5%. Thereby SbRGA114 can be used for candidate gene based association analysis and if validated, will be useful for improving Rabi sorghum for charcoal rot resistance through molecular breeding.
Total of 31 polymorphic EST-SSR markers which were developed and mapped at IABT UAS-Dharwad was used for association analysis. Population structure was assigned based on molecular data in Structure v. 2.0 software, while association analysis was done by Tassel program using the Q matrix. Six markers (Xiabt 210, Xiabt 527, Xiabt 301, Xiabt 37, Xiabt 77, Xiabt 81) for charcoal rot component traits and thirteen (Xiabt 82, Xiabt 85, Xiabt 47, Xiabt 30, Xiabt 241, Xiabt 457, Xiabt 275, Xiabt 397, Xiabt 378, Xiabt 380, Xiabt 445, Xiabt 81 and Xiabt 527) markers for yield component traits were stable at two locations viz., MARS Dharwad and RARS, Vijayapura. Also in the present study, previously identified flanking markers for charcoal rot disease (locus Xiabt 275 at RARS Vijayapura location) and yield related QTLs (Xiabt 81 and Xiabt 527 both MARS Dharwad and RARS Vijayapura locations) were validated. Therefore these markers can be utilised in marker assisted selection in charcoal rot disease resistance and yield improvement breeding programs.