Studies on Ping Pigmented Facultative Methylotrophs of Major Chilli Growing Areas of North Karnataka
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Date
2013
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UAS Dharwad
Abstract
Investigations were carried out to study the plant growth promotional ability and
management of anthracnose by native pink pigmented facultative methylotrophs (PPFMs) of
major chilli (Capsicum annuum L.) growing areas of North Karnataka. As many as 200
PPFM isolates were isolated and subjected to rapid screening for antibiosis against
Colletotrichum capsici (C. capsici) and qualitative assay for production of Indole acetic acid
(IAA). They were characterized based on morphological and biochemical characteristics.
Selected isolates were further screened for beneficial characters. Highest IAA and GA
production was recorded in PPFM6 (19.77 and 128.28 μg/ml of culture filtrate respectively)
and highest zeatin production was recorded in PPFM170 (2.54 ıg/ml). Some of the isolates
were positive for phosphate solubilization and antagonistic against Sclerotium rolfsii and
Fusarium oxysporum. These antagonistic isolates produced catechol type of siderophores
whereas production of HCN and volatile antibiotics was not displayed.
Based on in vitro studies, six promising isolates were selected for pot culture
experiments. The pathogen (C. capsici) was challenge inoculated and ISR activity was
estimated. The defence molecules tested were significantly influenced by PPFM isolates. The
peroxidase and PALase activity was increased from one DAI up to 7 DAI whereas chitinase,
PPO and phenol contents increased up to 5 DAI, after which gradual decrease was noticed.
Inoculation of PPFM isolates to chilli crop significantly improved plant height, dry matter
and chlorophyll content. Significantly lesser disease incidence, more yield and more
capsaicin content was observed with application of carbendazim followed by inoculation of
PPFM6 + PPFM170 + PPFM35 and PPFM6 both under pot culture and field conditions. The
results of the present study clearly indicated that PPFM6 is a very good plant growth
promoter and have biocontrol efficiency against C. capsici.