PHENOTYPIC AND MOLECULAR CHARACTERIZATION OF STAPHYLOCOCCAL CASSETTE CHROMOSOME MEC (SCCMEC) TYPES OF METHICILLIN-RESISTANT STAPHYLOCOCCI FROM ANIMAL AND HUMAN ORIGIN 2513
Loading...
Date
2018-03
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
JAU,JUNAGADH
Abstract
The present study was carried out with the objectives of isolation, identification and molecular characterization of Staphylococcal Cassette Chromosome mec (SCCmec) types along with antimicrobial resistance patterns and presence of virulent genes (toxic genes) in methicillin-resistant staphylococci from animals and humans. Out of 202 animals and 100 human nasal swabs, 86 (42.57%) and 62 (62%) isolates were Staphylococcus spp., respectively based on biochemical and molecular based identification. The antibiogram study revealed higher rates of methicillin, gentamicin, ofloxacin and levofloxacin sensitivity to human isolates, whereas higher susceptibility to amikacin and rifampicin followed by oxytetracyclin and chloramphenicol were observed in animal isolates. Out of total 86 and 62 staphylococci isolates, 74 and 50 isolates were Coagulase Negative Staphylococci (CoNS), 12 (from each) were Coagulase Positive Staphylococci (CoPS). Of these total Staphylococci isolated from both the species, 9 and 20 isolates were identified as Methicillin-Resistant Staphylococci (MRS) from animal and humans, respectively. Out of these MRS isolates, 8 and 18 were Methicillin-Resistant Coagulase Negative Staphylococci (MRCoNS), 1 and 2 isolates were Methicillin-Resistant Coagulase Positive Staphylococci (MRCoPS) from animal and humans, respectively. One isolate was identified as Methicillin-Resistant Coagulase Negative Staphylococcus aureus (MRCoNSA) from the animals and 2 isolates were identified as MRSA from humans. Of these 2 isolates, one isolate was Methicillin-Resistant Coagulase Negative Staphylococcus aureus (MRCoNSA) and one isolate was Methicillin-Resistant Coagulase Positive Staphylococcus aureus (MRCoPSA).
The study conducted for presence of virulence genes and their SCCmec typing on total 235 Staphylococcus spp. including departmental isolates which included, 148 Staphylococcus spp. (86 from animal milk/pus samples and 62 from human nasal swabs) from present study and 87 Staphylococcus spp. (47 from animal milk/pus samples and 40 from human nasal swabs) from departmental isolates. Out of these, 16 (12.03%) from animals and 40 (39.21%) from humans were having mecA gene which were classified as Methicillin-resistant staphylococci. Of these, 3 (2.26%) different isolates were found positive for all these virulence genes i.e. PVL, hla and icaA from
Abstract…
animals, whereas 7 (6.86%), 6 (5.88%) and 4 (3.92%) isolates were found positive for PVL, hla and icaA gene from humans, respectively. The SCCmec typing of MRS isolates were studied from 16 animals MRS isolates, of these, 14 isolates having one of the SCCmec types (SCCmec type I, 2; SCCmec type II, 0; SCCmec type III, 1; SCCmec type IV, 5; SCCmec type V, 6), whereas 2 isolates were Untypable. Out of total 40 humans MRS isolates, 28 isolates having one of the SCCmec types (SCCmec type I, 7; SCCmec type II, 0; SCCmec type III, 3; SCCmec type IV, 9; SCCmec type V, 9), whereas 12 isolates were Untypable. Based on SCCmec typing, 18.75% (3/16) and 25% (10/40) isolates were classified as hospital associated methicillin-resistant staphylococci (HA-MRS), whereas 68.75% (11/16) and 45% (18/40) isolates were classified as community associated methicillin resistant staphylococci (CA-MRS) from animal and humans, respectively. The overall percentage of CA-MRS (63.04%) was higher as compare to HA-MRS (28.26%) among both the species.
The detection of SCCmec types IV and V suggested the prevailed of CA-MRSA strains in this geographical area and occurrence of SCCmec I and II alleles indicated a possible transmission of MRSA from human to animals. The prevailed of same SCCmec types among animal and humans attribute to transmission of MRS from animal to human or vice versa indicating potential zoonotic pathogen prevalence in farm and farm workers.
Description
Keywords
VETERINARY MICROBIOLOGY