INDUCED MUTAGENESIS IN SESAME (Sesamum indicum L.)

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Date
2017
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Department of Plant Breeding and Genetics Anand Agricultural University Anand
Abstract
The present investigation “Induced mutagenesis in sesame (Sesamum indicum L.)” was undertaken to find out the variability induced by different doses of Ethyl Methane Sulfonate (EMS). The study was carried out at College Farm, B. A. College of Agriculture, Anand Agricultural University, Anand. The seeds of three entries viz. Gujarat Til-4 (GT-4), Gujarat Til -10 (GT-10) and Patan-64 were treated with 0.5%, 1.0%, 1.5%, 2.0% and 2.5% doses of EMS. The M1 generation was raised during summer season (2016) and harvested in bulk whereas M2 generation was raised during kharif season (2016) in Randomized block Design (factorial) with three replications. Mutagenic effect of EMS on seed germination with respect to different doses was recorded in M1 generation, where gradual reduction of germination per cent was noted with the increase in dose. In M2 generation observations were recorded for twelve characters viz. days to 50 per cent flowering, plant height, number of primary branches, number of capsule per plant, number of seed per capsule, capsule length, days to maturity, 1000 seed weight, yield per plant, harvest index, oil content and protein content. The objective of present investigation was to study the effect of chemical mutagen (EMS) on three genotypes, to estimate genotypic coefficient of variation (GCV), phenotypic coefficient of variation (PCV), heritability, genetic advance (GA) and also the genotypic and phenotypic correlations among different traits.
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