Management of Lentil Wilt Caused by Fusarium oxysporum f. sp. lentis

Thumbnail Image
Journal Title
Journal ISSN
Volume Title
Lentil is one of the major legume crops and play very important role in supply of protein to under nourished vegetarian population of the country. Lentil wilt caused by Fuasrium oxysporum f. sp. lentis is one of the main limiting factors for successful cultivation of lentil in India, causing significant economic losses. Lentil wilt symptoms recorded from infected plants was gradually drying of plants from the seedling stage up to flowering and late pod filling stage. Symptoms as leaves turn yellow and curling of leaves begins from the lower end and extends up wards. Then crown droops and this is followed by the death of the plant. The root system was developed poorly and discolored brown. The fungus was isolated and purified from wilted lentil plant collected from field of Agriculture Research Station Ummedganj, Kota and found pathogenic to cause lentil will. Further, identified as Fusarium oxysporum f,sp lentis by ITCC, Division of Plant Pathology IARI, New Delhi. Mycelium growth and sporulation were maximum on oat meal agar (Hi-media) medium was statistically at par with potato dextrose agar (Himedia), epidemiological requirement of pathogen was 30 ℃ temperature & pH level 6.0 under lab experiment. Result revealed that none of entries are immune and highly resistant (HR), 11 entries were resistant (R), 5 entries are moderately resistant (MR), 4 entries as susceptible (S), While, no one among these entries were recorded as highly susceptible (HS). Four species of Trichoderma were tested against Fusarium oxysporum f. sp. lentis dual culture technique inoculated at different time of interval, T. viride found most effective for growth inhibition of pathogen in all the bio-agents tested in dual culture with reference the time interval. Result also indicated that, the pathogen inoculated 96 hrs. before bioagent inoculation gave good antagonistic reaction in dual culture technique. Out of seven systemic and non-systemic fungicides, most effective was found Carbendazim 50% WP, Propiconazole 25% EC inhibited completely the mycelial growth of Fusarium oxysporum f. sp. lentis at all concentrations tested, it was statistically at par with hexaconazole 5% EC at 125 & 200 ppm respectively. Under pot experiment standardization of inoculum level maximum lentil wilt incidence was observed in pots where mass culture added @ 10 g/kg soil of pot (89.09%) whereas, minimum lentil wilt incidence (47.42%) was recorded in pots where mass culture added @ 2 g/kg soil of pot. Concluded that inoculum density is bound to influence the disease incidence. Bioagents applied through seed treatment @ 0.6% spore suspension / kg seeds (1x108 spore /ml) for manage lentil wilt. Trichoderma viride showed more protection against lentil wilt and recorded more yield followed by Trichoderma harzianum. Trichoderma spp. had synergistic impact on plant vigour and grain yield. Carbendazim 50% WP applied through seed treatment @ 0.2% / kg seeds gave maximum protection against lentil wilt, it was followed by hexaconazole 5% EC in pot experiment.
Management of Lentil Wilt Caused by Fusarium oxysporum f. sp. lentis