GENETIC AND MOLECULAR ANALYSIS IN VEGETABLE AND GRAIN TYPE SOYBEAN (Glycine max (L.) Merrill).

dc.contributor.advisorNandanwar, Dr. R. S. Dr. R. S.
dc.contributor.advisorNandanwar, Dr. R. S. Dr. R. S.
dc.contributor.advisorNandanwar, Dr. R. S. Dr. R. S.
dc.contributor.authorSONKAMBLE, PRITI ARVIND.
dc.contributor.authorSONKAMBLE, PRITI ARVIND.
dc.contributor.authorSONKAMBLE, PRITI ARVIND.
dc.contributor.authorSONKAMBLE, PRITI ARVIND.
dc.date.accessioned2023-01-01T20:21:31Z
dc.date.available2023-01-01T20:21:31Z
dc.date.issued2021-01-01
dc.date.issued2021-01-01
dc.date.issued2021-01-01
dc.date.issued2021-01-01
dc.descriptionThe present investigation was carried out at the Department of Agricultural Botany, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola during 2016-2019. The research was undertaken to characterize and analyze the proximate composition of fourteen soybean genotypes including seven of grain and vegetable types and the genetic inheritance of eight contrasting characters of five promising crosses.en_US
dc.descriptionThe present investigation was carried out at the Department of Agricultural Botany, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola during 2016-2019. The research was undertaken to characterize and analyze the proximate composition of fourteen soybean genotypes including seven of grain and vegetable types and the genetic inheritance of eight contrasting characters of five promising crosses.en_US
dc.descriptionThe present investigation was carried out at the Department of Agricultural Botany, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola during 2016-2019. The research was undertaken to characterize and analyze the proximate composition of fourteen soybean genotypes including seven of grain and vegetable types and the genetic inheritance of eight contrasting characters of five promising crosses.en_US
dc.descriptionThe present investigation was carried out at the Department of Agricultural Botany, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola during 2016-2019. The research was undertaken to characterize and analyze the proximate composition of fourteen soybean genotypes including seven of grain and vegetable types and the genetic inheritance of eight contrasting characters of five promising crosses.en_US
dc.description.abstractThe present investigation entitled “Genetic and molecular analysis in vegetable and grain type soybean (Glycine max (L.) Merrill)” was undertaken to characterize and analyse proximate composition of vegetable and grain type soybean genotypes, genetic inheritance of contrasting characters and characterization of promising crosses at molecular level. Morphological characterization and genetic variability studies were carried out using fourteen soybean genotypes including seven each of grain and vegetable types during Kharif 2016-17. The experimental populations of five different crosses (F1’s, backcrosses with both parents and F2) were developed during Kharif 2016-17 to 2017-18 for genetic inheritance studies. Evaluation of all the populations along with their parents and molecular work was carried out at experimental field of Department of Agricultural Botany and Biotechnology Centre, Dr. PDKV, Akola, respectively during 2018-19. Ten characters were recorded for morphological characterization of soybean genotypes and categorized based on the expression of distinguishable morphological traits with two to four expressions. Analysis of variance indicated significant differences among the genotypes for different traits. The phenotypic coefficient of variation (PCV) was higher than genotypic coefficient of variation (GCV). The high values of GCV and PCV observed for 100 green seed (bean) weight, 100 seed (matured) weight, seed yield per plant, green seed (bean) yield per plant and harvest index. High heritability coupled with high genetic advance was observed for days to 50% flowering, days to maturity, plant height, number of branches per plant, number of seed per pod, green pod weight per plant, 100 green seed (bean), 100 seed weight, green seed (bean) yield per plant, seed yield per plant, harvest index, oil content, protein content, iron content, zinc content and trypsin inhibitor content showed preponderance of additive gene effects in expression of these traits. Among the soybean genotypes, Swarna Vasundhara was found promising for green pod weight, seed yield per plant, harvest index and protein content at R6 stage. The genotype AGS-450 exhibited high iron with low trypsin content whereas, AGS-459 exhibited high sugar and zinc content with aroma, soft texture and sweet taste. Eight contrasting characters (leaf shape, flower colour, pod pubescence, pod pubescence colour, pod colour, plant height, seed colour and seed shape) used for genetic inheritance studies. All contrasting traits were governed by single gene with complete dominance, except leaf shape in crosses Swarna Vasundhara X JS(SH)93-37 and AGS-339 X JS(SH)93-37, plant height in three crosses viz. Swarna Vasundhara X JS(SH)93-37, GC-84501-32-01 X JS(SH)93-37and AGS-450 X JS(SH)93-37 and seed shape in cross Swarna Vasundhara X JS(SH)93-37 showed complementary gene interaction (9:7). Plant height also showed duplicate gene interaction (15:1) in cross AGS-339 X JS(SH)93-37 and seed colour in cross Swarna Vasundhara X JS(SH)93-37 showed supplementary gene interaction (9:3:4) indicating two genes were responsible for these traits. Characterization of fourteen soybean genotypes and six parents along with sixteen selected desirable lines of five crosses carried out by using molecular markers. Among soybean genotypes, thirteen SSR primers found polymorphic and the similarity matrix coefficient ranged from 0.323 to 0.905. Eleven SSR primers (Simple sequence repeat) were found to be polymorphic among the six parents and their selected lines and the similarity matrix coefficient ranged from 0.310 to 0.818. Dendrogram analysis depicted six clusters with huge genetic diversity in all soybean genotypes including vegetable and grain type whereas, three clusters in parents and desirable lines at molecular level. Present investigation on characterization and genetic variability can be used to measure the extent of variation in various morphological characters, transmissibility of character from parent to their offspring and genetic gain from the selection applied in population. Genetic inheritance studies can guide soybean breeders for transfer of contrasting traits in desirable genotypes by using appropriate breeding strategy and molecular characterization study will be helpful in identification of genetically diverse soybean genotypes, parents and advanced lines for broadening the genetic base and improving the breeding efficiency.en_US
dc.description.abstractThe present investigation entitled “Genetic and molecular analysis in vegetable and grain type soybean (Glycine max (L.) Merrill)” was undertaken to characterize and analyse proximate composition of vegetable and grain type soybean genotypes, genetic inheritance of contrasting characters and characterization of promising crosses at molecular level. Morphological characterization and genetic variability studies were carried out using fourteen soybean genotypes including seven each of grain and vegetable types during Kharif 2016-17. The experimental populations of five different crosses (F1’s, backcrosses with both parents and F2) were developed during Kharif 2016-17 to 2017-18 for genetic inheritance studies. Evaluation of all the populations along with their parents and molecular work was carried out at experimental field of Department of Agricultural Botany and Biotechnology Centre, Dr. PDKV, Akola, respectively during 2018-19. Ten characters were recorded for morphological characterization of soybean genotypes and categorized based on the expression of distinguishable morphological traits with two to four expressions. Analysis of variance indicated significant differences among the genotypes for different traits. The phenotypic coefficient of variation (PCV) was higher than genotypic coefficient of variation (GCV). The high values of GCV and PCV observed for 100 green seed (bean) weight, 100 seed (matured) weight, seed yield per plant, green seed (bean) yield per plant and harvest index. High heritability coupled with high genetic advance was observed for days to 50% flowering, days to maturity, plant height, number of branches per plant, number of seed per pod, green pod weight per plant, 100 green seed (bean), 100 seed weight, green seed (bean) yield per plant, seed yield per plant, harvest index, oil content, protein content, iron content, zinc content and trypsin inhibitor content showed preponderance of additive gene effects in expression of these traits. Among the soybean genotypes, Swarna Vasundhara was found promising for green pod weight, seed yield per plant, harvest index and protein content at R6 stage. The genotype AGS-450 exhibited high iron with low trypsin content whereas, AGS-459 exhibited high sugar and zinc content with aroma, soft texture and sweet taste. Eight contrasting characters (leaf shape, flower colour, pod pubescence, pod pubescence colour, pod colour, plant height, seed colour and seed shape) used for genetic inheritance studies. All contrasting traits were governed by single gene with complete dominance, except leaf shape in crosses Swarna Vasundhara X JS(SH)93-37 and AGS-339 X JS(SH)93-37, plant height in three crosses viz. Swarna Vasundhara X JS(SH)93-37, GC-84501-32-01 X JS(SH)93-37and AGS-450 X JS(SH)93-37 and seed shape in cross Swarna Vasundhara X JS(SH)93-37 showed complementary gene interaction (9:7). Plant height also showed duplicate gene interaction (15:1) in cross AGS-339 X JS(SH)93-37 and seed colour in cross Swarna Vasundhara X JS(SH)93-37 showed supplementary gene interaction (9:3:4) indicating two genes were responsible for these traits. Characterization of fourteen soybean genotypes and six parents along with sixteen selected desirable lines of five crosses carried out by using molecular markers. Among soybean genotypes, thirteen SSR primers found polymorphic and the similarity matrix coefficient ranged from 0.323 to 0.905. Eleven SSR primers (Simple sequence repeat) were found to be polymorphic among the six parents and their selected lines and the similarity matrix coefficient ranged from 0.310 to 0.818. Dendrogram analysis depicted six clusters with huge genetic diversity in all soybean genotypes including vegetable and grain type whereas, three clusters in parents and desirable lines at molecular level. Present investigation on characterization and genetic variability can be used to measure the extent of variation in various morphological characters, transmissibility of character from parent to their offspring and genetic gain from the selection applied in population. Genetic inheritance studies can guide soybean breeders for transfer of contrasting traits in desirable genotypes by using appropriate breeding strategy and molecular characterization study will be helpful in identification of genetically diverse soybean genotypes, parents and advanced lines for broadening the genetic base and improving the breeding efficiency.en_US
dc.description.abstractThe present investigation entitled “Genetic and molecular analysis in vegetable and grain type soybean (Glycine max (L.) Merrill)” was undertaken to characterize and analyse proximate composition of vegetable and grain type soybean genotypes, genetic inheritance of contrasting characters and characterization of promising crosses at molecular level. Morphological characterization and genetic variability studies were carried out using fourteen soybean genotypes including seven each of grain and vegetable types during Kharif 2016-17. The experimental populations of five different crosses (F1’s, backcrosses with both parents and F2) were developed during Kharif 2016-17 to 2017-18 for genetic inheritance studies. Evaluation of all the populations along with their parents and molecular work was carried out at experimental field of Department of Agricultural Botany and Biotechnology Centre, Dr. PDKV, Akola, respectively during 2018-19. Ten characters were recorded for morphological characterization of soybean genotypes and categorized based on the expression of distinguishable morphological traits with two to four expressions. Analysis of variance indicated significant differences among the genotypes for different traits. The phenotypic coefficient of variation (PCV) was higher than genotypic coefficient of variation (GCV). The high values of GCV and PCV observed for 100 green seed (bean) weight, 100 seed (matured) weight, seed yield per plant, green seed (bean) yield per plant and harvest index. High heritability coupled with high genetic advance was observed for days to 50% flowering, days to maturity, plant height, number of branches per plant, number of seed per pod, green pod weight per plant, 100 green seed (bean), 100 seed weight, green seed (bean) yield per plant, seed yield per plant, harvest index, oil content, protein content, iron content, zinc content and trypsin inhibitor content showed preponderance of additive gene effects in expression of these traits. Among the soybean genotypes, Swarna Vasundhara was found promising for green pod weight, seed yield per plant, harvest index and protein content at R6 stage. The genotype AGS-450 exhibited high iron with low trypsin content whereas, AGS-459 exhibited high sugar and zinc content with aroma, soft texture and sweet taste. Eight contrasting characters (leaf shape, flower colour, pod pubescence, pod pubescence colour, pod colour, plant height, seed colour and seed shape) used for genetic inheritance studies. All contrasting traits were governed by single gene with complete dominance, except leaf shape in crosses Swarna Vasundhara X JS(SH)93-37 and AGS-339 X JS(SH)93-37, plant height in three crosses viz. Swarna Vasundhara X JS(SH)93-37, GC-84501-32-01 X JS(SH)93-37and AGS-450 X JS(SH)93-37 and seed shape in cross Swarna Vasundhara X JS(SH)93-37 showed complementary gene interaction (9:7). Plant height also showed duplicate gene interaction (15:1) in cross AGS-339 X JS(SH)93-37 and seed colour in cross Swarna Vasundhara X JS(SH)93-37 showed supplementary gene interaction (9:3:4) indicating two genes were responsible for these traits. Characterization of fourteen soybean genotypes and six parents along with sixteen selected desirable lines of five crosses carried out by using molecular markers. Among soybean genotypes, thirteen SSR primers found polymorphic and the similarity matrix coefficient ranged from 0.323 to 0.905. Eleven SSR primers (Simple sequence repeat) were found to be polymorphic among the six parents and their selected lines and the similarity matrix coefficient ranged from 0.310 to 0.818. Dendrogram analysis depicted six clusters with huge genetic diversity in all soybean genotypes including vegetable and grain type whereas, three clusters in parents and desirable lines at molecular level. Present investigation on characterization and genetic variability can be used to measure the extent of variation in various morphological characters, transmissibility of character from parent to their offspring and genetic gain from the selection applied in population. Genetic inheritance studies can guide soybean breeders for transfer of contrasting traits in desirable genotypes by using appropriate breeding strategy and molecular characterization study will be helpful in identification of genetically diverse soybean genotypes, parents and advanced lines for broadening the genetic base and improving the breeding efficiency.en_US
dc.description.abstractThe present investigation entitled “Genetic and molecular analysis in vegetable and grain type soybean (Glycine max (L.) Merrill)” was undertaken to characterize and analyse proximate composition of vegetable and grain type soybean genotypes, genetic inheritance of contrasting characters and characterization of promising crosses at molecular level. Morphological characterization and genetic variability studies were carried out using fourteen soybean genotypes including seven each of grain and vegetable types during Kharif 2016-17. The experimental populations of five different crosses (F1’s, backcrosses with both parents and F2) were developed during Kharif 2016-17 to 2017-18 for genetic inheritance studies. Evaluation of all the populations along with their parents and molecular work was carried out at experimental field of Department of Agricultural Botany and Biotechnology Centre, Dr. PDKV, Akola, respectively during 2018-19. Ten characters were recorded for morphological characterization of soybean genotypes and categorized based on the expression of distinguishable morphological traits with two to four expressions. Analysis of variance indicated significant differences among the genotypes for different traits. The phenotypic coefficient of variation (PCV) was higher than genotypic coefficient of variation (GCV). The high values of GCV and PCV observed for 100 green seed (bean) weight, 100 seed (matured) weight, seed yield per plant, green seed (bean) yield per plant and harvest index. High heritability coupled with high genetic advance was observed for days to 50% flowering, days to maturity, plant height, number of branches per plant, number of seed per pod, green pod weight per plant, 100 green seed (bean), 100 seed weight, green seed (bean) yield per plant, seed yield per plant, harvest index, oil content, protein content, iron content, zinc content and trypsin inhibitor content showed preponderance of additive gene effects in expression of these traits. Among the soybean genotypes, Swarna Vasundhara was found promising for green pod weight, seed yield per plant, harvest index and protein content at R6 stage. The genotype AGS-450 exhibited high iron with low trypsin content whereas, AGS-459 exhibited high sugar and zinc content with aroma, soft texture and sweet taste. Eight contrasting characters (leaf shape, flower colour, pod pubescence, pod pubescence colour, pod colour, plant height, seed colour and seed shape) used for genetic inheritance studies. All contrasting traits were governed by single gene with complete dominance, except leaf shape in crosses Swarna Vasundhara X JS(SH)93-37 and AGS-339 X JS(SH)93-37, plant height in three crosses viz. Swarna Vasundhara X JS(SH)93-37, GC-84501-32-01 X JS(SH)93-37and AGS-450 X JS(SH)93-37 and seed shape in cross Swarna Vasundhara X JS(SH)93-37 showed complementary gene interaction (9:7). Plant height also showed duplicate gene interaction (15:1) in cross AGS-339 X JS(SH)93-37 and seed colour in cross Swarna Vasundhara X JS(SH)93-37 showed supplementary gene interaction (9:3:4) indicating two genes were responsible for these traits. Characterization of fourteen soybean genotypes and six parents along with sixteen selected desirable lines of five crosses carried out by using molecular markers. Among soybean genotypes, thirteen SSR primers found polymorphic and the similarity matrix coefficient ranged from 0.323 to 0.905. Eleven SSR primers (Simple sequence repeat) were found to be polymorphic among the six parents and their selected lines and the similarity matrix coefficient ranged from 0.310 to 0.818. Dendrogram analysis depicted six clusters with huge genetic diversity in all soybean genotypes including vegetable and grain type whereas, three clusters in parents and desirable lines at molecular level. Present investigation on characterization and genetic variability can be used to measure the extent of variation in various morphological characters, transmissibility of character from parent to their offspring and genetic gain from the selection applied in population. Genetic inheritance studies can guide soybean breeders for transfer of contrasting traits in desirable genotypes by using appropriate breeding strategy and molecular characterization study will be helpful in identification of genetically diverse soybean genotypes, parents and advanced lines for broadening the genetic base and improving the breeding efficiency.en_US
dc.identifier.citationSONKAMBLE, PRITI ARVIND. (2020). Genetic and molecular analysis in vegetable and grain type soybean (Glycine max (L.) Merrill). Department of Agricultural Botany. Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola. Ph. D. 2020. Print. xx, 224p. (Unpublished).en_US
dc.identifier.citationSONKAMBLE, PRITI ARVIND. (2020). Genetic and molecular analysis in vegetable and grain type soybean (Glycine max (L.) Merrill). Department of Agricultural Botany. Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola. Ph. D. 2020. Print. xx, 224p. (Unpublished).en_US
dc.identifier.citationSONKAMBLE, PRITI ARVIND. (2020). Genetic and molecular analysis in vegetable and grain type soybean (Glycine max (L.) Merrill). Department of Agricultural Botany. Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola. Ph. D. 2020. Print. xx, 224p. (Unpublished).en_US
dc.identifier.citationSONKAMBLE, PRITI ARVIND. (2020). Genetic and molecular analysis in vegetable and grain type soybean (Glycine max (L.) Merrill). Department of Agricultural Botany. Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola. Ph. D. 2020. Print. xx, 224p. (Unpublished).en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810190959
dc.keywordsGlycine max, Soybean, Soybean genotypes, Gene interaction soybean, F1 soybean, Soybean morphological characterization, Self-pollination Soybean, Soybean hybridization, Soybean genetic inheritance, Soybean genetic variability, Soybean nutritional quality, Soybean molecular marker, Soybean SSR polymorphism.en_US
dc.keywordsGlycine max, Soybean, Soybean genotypes, Gene interaction soybean, F1 soybean, Soybean morphological characterization, Self-pollination Soybean, Soybean hybridization, Soybean genetic inheritance, Soybean genetic variability, Soybean nutritional quality, Soybean molecular marker, Soybean SSR polymorphism.en_US
dc.keywordsGlycine max, Soybean, Soybean genotypes, Gene interaction soybean, F1 soybean, Soybean morphological characterization, Self-pollination Soybean, Soybean hybridization, Soybean genetic inheritance, Soybean genetic variability, Soybean nutritional quality, Soybean molecular marker, Soybean SSR polymorphism.en_US
dc.keywordsGlycine max, Soybean, Soybean genotypes, Gene interaction soybean, F1 soybean, Soybean morphological characterization, Self-pollination Soybean, Soybean hybridization, Soybean genetic inheritance, Soybean genetic variability, Soybean nutritional quality, Soybean molecular marker, Soybean SSR polymorphism.en_US
dc.language.isoEnglishen_US
dc.language.isoEnglishen_US
dc.language.isoEnglishen_US
dc.language.isoEnglishen_US
dc.pagesxx, 224p.en_US
dc.pagesxx, 224p.en_US
dc.pagesxx, 224p.en_US
dc.pagesxx, 224p.en_US
dc.publisherDr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, Maharashtra.en_US
dc.publisherDr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, Maharashtra.en_US
dc.publisherDr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, Maharashtra.en_US
dc.publisherDr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, Maharashtra.en_US
dc.research.problemIn the present research the researcher has tried to study the characterization and proximate composition analysis of vegetable and grain type soybean genotypes and to determine inheritance of targeted quality and quantitative traits in soybean population and also tried to study molecular characterization of promising crosses using genic markers and assessment of nutritional quality and value added traits.en_US
dc.research.problemIn the present research the researcher has tried to study the characterization and proximate composition analysis of vegetable and grain type soybean genotypes and to determine inheritance of targeted quality and quantitative traits in soybean population and also tried to study molecular characterization of promising crosses using genic markers and assessment of nutritional quality and value added traits.en_US
dc.research.problemIn the present research the researcher has tried to study the characterization and proximate composition analysis of vegetable and grain type soybean genotypes and to determine inheritance of targeted quality and quantitative traits in soybean population and also tried to study molecular characterization of promising crosses using genic markers and assessment of nutritional quality and value added traits.en_US
dc.research.problemIn the present research the researcher has tried to study the characterization and proximate composition analysis of vegetable and grain type soybean genotypes and to determine inheritance of targeted quality and quantitative traits in soybean population and also tried to study molecular characterization of promising crosses using genic markers and assessment of nutritional quality and value added traits.en_US
dc.subGenetics and Plant Breedingen_US
dc.subGenetics and Plant Breedingen_US
dc.subGenetics and Plant Breedingen_US
dc.subGenetics and Plant Breedingen_US
dc.themeThe present research deals with the Characterization of all soybean genotypes, genetic inheritance and promising crosses along with their parents at molecular level was studied through SSR polymorphism.en_US
dc.themeThe present research deals with the Characterization of all soybean genotypes, genetic inheritance and promising crosses along with their parents at molecular level was studied through SSR polymorphism.en_US
dc.themeThe present research deals with the Characterization of all soybean genotypes, genetic inheritance and promising crosses along with their parents at molecular level was studied through SSR polymorphism.en_US
dc.themeThe present research deals with the Characterization of all soybean genotypes, genetic inheritance and promising crosses along with their parents at molecular level was studied through SSR polymorphism.en_US
dc.these.typePh.Den_US
dc.these.typePh.Den_US
dc.these.typePh.Den_US
dc.these.typePh.Den_US
dc.titleGENETIC AND MOLECULAR ANALYSIS IN VEGETABLE AND GRAIN TYPE SOYBEAN (Glycine max (L.) Merrill).en_US
dc.titleGENETIC AND MOLECULAR ANALYSIS IN VEGETABLE AND GRAIN TYPE SOYBEAN (Glycine max (L.) Merrill).en_US
dc.titleGENETIC AND MOLECULAR ANALYSIS IN VEGETABLE AND GRAIN TYPE SOYBEAN (Glycine max (L.) Merrill).en_US
dc.titleGENETIC AND MOLECULAR ANALYSIS IN VEGETABLE AND GRAIN TYPE SOYBEAN (Glycine max (L.) Merrill).en_US
dc.typeThesisen_US
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The present investigation was carried out at Department of Agricultural Botany, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola during 2016-2019. The research was undertaken to characterize and analyse proximate composition of fourteen soybean genotypes including seven each of grain and vegetable types and genetic inheritance of eight contrasting characters of five promising crosses.
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