Studies on induction of radiomutants for novel traits in Chrysanthemum (Chrysanthemum morifolium Ramat.)
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Date
2016
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Punjab Agricultural University, Ludhiana
Abstract
The research entitled “Studies on induction of radiomutants for novel traits in Chrysanthemum (Chrysanthemum morifolium Ramat.)” was conducted at Research Farm, Department of Floriculture and Landscaping, Punjab Agricultural University, Ludhiana, during the year 2015-16. The terminal rooted cuttings of two chrysanthemum cvs. ‘Thai Chen Queen’ and ‘Purnima’ were treated with 0, 5, 10, 15 and 20 Gy dose of gamma rays and transplanted in pots (8") immediately after treatment. The experiment was conducted in factorial completely randomized design (FCRD) with four replications comprising twenty five pots per replication. Gamma ray treated rooted cuttings along with control plants were evaluated for various vegetative and flowering characters. The maximum plant survival (70.44 %) was recorded in untreated plants and reduction in plant survival was increased with increasing dose of gamma irradiation. LD50 dose was determined as 20 Gy dose for cv. Thai Chen Queen and 15 Gy for cv. Purnima. In both the cultivars vegetative growth parameters decreases with increasing dose of gamma irradiation. Plant height at bud appearance (41.19 cm), (33.15 cm) and Internodal length (0.90 cm), (0.70 cm) was much reduced when rooted cuttings are treated with 20 Gy in both the cvs. Thai Chen Queen and Purnima respectively. The treatment with 20 Gy of gamma rays delayed bud initiation as well as flowering in both cultivars. Flower size and number of ray florets also decreased with increasing gamma irradiation. The leaf abnormalities were observed in terms of change in leaf shape, leaf size and floral abnormalities in terms of flower form which increased as the dose of gamma irradiation increased. Marked variations were recorded between the mutated and control populations. In cv. ‘Thai Chen Queen’ seven flower colour variants are developed, three at 10 Gy, three at 15 Gy and other one at 20 Gy whereas, two flower colour variants were developed in cv. ‘Purnima’. One at 10 Gy and one flower colour variants was developed at 15 Gy. This study developed a mutagenesis protocol that could be used to develop novel colour mutants in chrysanthemum.
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