TAGGING OF GENE FOR RESISTANCE TO POST FLOWERING STALK ROT IN MAIZE(Zea mays) CAUSED BY Macrophomina phaseolina
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Date
2010
Authors
Journal Title
Journal ISSN
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ACHARYA N.G. RANGA AGRICULTURAL UNIVERSITY RAJENDRANAGAR, HYDERABAD
Abstract
Maize is one of the most important economic cereal crops and an ideal forage
crop. It occupies a prominent position in global agriculture. 67% of maize is used for
live stock feed and 25% of maize for human consumption, industrial purposes and
the balance is used as seed.The productivity of maize is influenced by both biotic and
abiotic factors. Maize suffers from about 110 diseases on a global basis.
In India there are four downy mildews, four stalk rots, three foliar diseases,
root rots and other diseases affecting kernel and other aerial parts. Disease spectrum
varies in different agro climatic zones. More serious diseases are leaf blight, stalk
rots, downy mildews and rusts. Stalk rots take a heavy toll, among which stalk rots
caused by Macrophomina phaseolina (Tassi) Goid and Fusarium moniliformae
results in 30-40% losses. Post flowering stalk rot (PFSR) is the complex disease
caused by three fungi,viz., Cephalosporium acremonium, Macrophomina phaseolina,
Fusarium moniliformae and one bacterium Erwinia carotovora var zeae. Out of
these, post flowering stalk rot caused by Macrophomina phaseolina is the important
disease of maize in the state of Andhra Pradesh.
Author : P.SUNEETHA
Title of the thesis : “TAGGING OF GENE FOR RESISTANCE TO
POST FLOWERING STALK ROT IN
MAIZE(Zea mays) CAUSED BY Macrophomina
phaseolina”
Degree to which it is
submitted
: MASTER OF SCIENCE IN AGRICULTURE
Faculty : AGRICULTURE
Department : AGRICULTURAL BIOTECHNOLOGY
Major Advisor : Dr. G.ANURADHA
Principal Scientist (Breeding)
Biotechnology Unit
Agricultural Research Institute
Rajendranagar, Hyderabad – 30.
University : ACHARYA N. G. RANGA AGRICULTURAL
UNIVERSITY
Year of submission : 2010
In order to tag the post flowering stalk rot resistant gene maize inbred lines
BPPTI-34 (resistant) and BPPTI -66(Susceptible), were crossed to produce F1. F1’s
were selfed as well as back crossed to the susceptible parent to derive F2 and BC1F1
populations respectively. Parents P1 and P2, F1 and two mapping populations F2 and
BC1F1 were artificially inoculated with the Macrophomina phaseolina culture. F1s
inoculated with the culture showed resistant reaction revealing for that resistance for
post flowering stalk rot is governed by dominant gene. F2 population segregated in
3:1 ratio i.e 87 resistant: 27 susceptible and BC1F1 population segregated in the ratio
of 1:1 i.e 26 resistant: 24 susceptible showing that resistance to post flowering stalk
rot is governed by single dominant gene.
A total of 150 microsatellite markers distributed on entire genome were used
to screen the parents. Of these, 54 SSR markers from ten chromosomes were found
polymorphic in the parents. These fifty four markers were used to screen the bulk
DNAs prepared from 10 plants each of resistant and susceptible plants from F2 and
BC1F1 populations to find the markers linked to the resistance gene. By bulked
segregant analysis (BSA) the marker umc 1269 clearly distinguished resistant and
susceptible bulks as that of resistant and susceptible parents indicating that this
marker is tightly linked to the gene for resistance to post flowering stalk rot.
Description
Keywords
TAGGING, GENE,RESISTANCE, POST, FLOWERING ,STALK, ROT, MAIZE, CAUSED, Macrophomina, phaseolina