Monitoring insecticide resistance in diamondback moth, Plutella xylostella (Linnaeus) in different locations of Punjab

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2012
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Diamondback moth (DBM), Plutella xylostella (Linnaeus) (Lepidoptera: Plutellidae) is a recurring global agricultural pest of cole crops in India and throughout the world. Toxicity of some insecticides against the susceptible Punjab population, maintained for over 30 generations and field populations of P. xylostella collected from vegetable growing areas of Amritsar, Ludhiana and Kapurthala in the Punjab were tested using leaf-disc dip technique. Based on the LC50 values, the susceptible population showed highest sensitivity to emamectin benzoate with lowest LC50 value of 0.00003 per cent while least for cartap hydrochloride (0.00145%). Chlorantraniliprole was the most toxic molecule against the field populations tested with LC50 values ranging from 0.00012 - 0.00027 per cent followed by emamectin benzoate (0.00154 - 0.00336%) while cartap hydrochloride was the least effective with LC50 value ranging from 0.03819 to 0.07662 per cent. DBM population of Amritsar was found to be the most resistant one for all the test insecticides except indoxacarb, where Ludhiana population lead in resistance. Field populations of P. xylostella showed maximum resistance against emamectin benzoate (51.3 to 112-fold) followed by cartap hydrochloride (26.33 to 52.84-fold) while minimum against chlorantraniliprole (2.4 to 5.4-fold).
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