Bioethanol production from starchy wastes

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Date
2017
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CCSHAU
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Potato (Solanum tuberosum) is one of the most important agricultural crops for human consumption and high amount is produced worldwide every year. Potatoes are starchy crops, which do not require complex pre-treatments. Potato peel waste (PPW) is a zero value by product, which occurs in big amounts after industrial potato processing and can range from 15-40 % of initial product mass, depending on the peeling method. PPW has potential as a base for fermentation reactions because of high starch content and could be utilized for bioethanol production. Sweet potato (Ipomoea batatas L.) represents an important biomass resource for fuel alcohol production, because of its chemical composition and high density of starch, compared to other forms of biomass, and thus premise as an alternative bioresource for the production of ethanol through fermentation. Ethanol production from potato and sweet potato peel wastes (SPPW) is relatively new topic and limited research has been conducted about the utilization of these wastes. In the present study, a total of 15 yeast isolates were obtained from soil and other sources like fruits and vegetables, fermented foods etc. Out of these, 12 yeast isolates were screened for amylolytic activity on starch agar medium. The zone index varied from 1.5 to 3.2 with yeast isolate YPO3 showing the maximum zone index (3.2). Only six isolates were selected for measuring the amylolytic enzyme activity. The maximum enzyme activity was observed in YPO3 (181.54 U/ml). However, four yeast isolates were used for optimization of conditions by SHF method for ethanol production from potato and sweet potato peel wastes at different temperatures (30, 35 and 40°C), pH (5.0, 6.0 and 7.0) and incubation period under stationary condition. The maximum bioethanol production was observed from PPW and SPPW at temperature 35°C and pH 6.0 after 72 h of incubation under SHF. SSF of PPW and SPPW was observed at temperature 35°C and pH 6.0 after 72 h of incubation period using YPO3 and YPmp3. The maximum ethanol 2.83% and 3.14 % were observed by using YPO3 after 72 h of incubation, whereas 3.75% and 4.62% were observed using Sacchromyces cerevisiae HAU-1 after 48 h of incubation from PPW and SPPW. The yeast isolates (YPmp3 and YPO3) showed maximum ethanol production in SSF as compared to SHF.
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