Characterization of Rhizobium isolates of Pigeon pea collected from the acidic soils of the state of Jharkhand.
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Date
2019
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Birsa Agricultural University, Ranchi
Abstract
Agriculture sector is the backbone of Indian economy however; agricultural
productivity is very low in some countries. The low productivity is due to decreasing
soil fertility many factors responsible for decreasing soil fertility such as soil acidity,
soil erosion, and continuous cropping. Soil acidity o.ccurs when there is a buildup of
acid forming element. Acidic soil in Jharkhand occupies approximately 90 percent area
of the land. Most leguminous plant requires neutral or slightly acidic soil for growth,
especially when depending on symbiotic nitrogen fixitation.
Legumes, improves soil fertility through biological nitrogen fixation. It is
reported to contribute approximately 40 kg N ha-1 soil acidity is one of the serious
problem which affect growth of Rhizobia in the soil of Jharkhand Rhizobium is a gram
negative bacteria, soil living bacteria. Rhizobium provides organic nitrogenous
compound to the plant and plant provide organic compound made by photosynthesis,
this relation is known as symbiosis.
The present study focuses on the proteomic analysis of Rhizobium isolates of
pigeon pea. This objective was achieved by performing the 2 DE using fourteen (14)
different isolate of Rhizobium collected from different regimes of state of Jharkhand.
We analysed unique spots with the help of Two Dimensional Gel Electrophoresis.
‘Unique' protein spots identified the genes implicated in the acid-soil tolerance. Two
Dimensional Polyacrylamide Gel Electrophoresis of proteins is a robust and
reproducible technique. It is the most widely used separation tool in proteomics, by
utilizing MALDI-TOF-TOF approach subsequently genes which might be implicated to
play crucial roles in imparting acid soil-tolerance to Rhizobium isolates of pigeon pea
were identified from the isolates collected acid soils of the state of Jharkhand various
spots of Rhizobium isolates were analyzed Protein play a major role to cope with
abiotic stress.
In this study we perform 2-DE electrophoresis to establish the reproducibility of
the protein and MALDI TOF/TOF analysis to identify gene identity of the protein spot.
Synthetic peptide was prepared against the protein spot which is expressed in the crop
in response to the acid-soil tolerance. Subsequently, antibody is produced by against
this peptide as a follow-up. This antibody is employed to perform the Western Blot
Analysis in order to detect the proteins induced specifically in response to the acid soil
tolerance regimes.
Description
Characterization of Rhizobium isolates of Pigeon pea collected from the acidic soils of the state of Jharkhand.