Effect of probiotics on the growth performance of Cyprinus carpio L. culture under biofloc technology/system
| dc.contributor.advisor | Dahiya, Tejpal | |
| dc.contributor.author | Poonam | |
| dc.date.accessioned | 2024-07-03T07:34:40Z | |
| dc.date.available | 2024-07-03T07:34:40Z | |
| dc.date.issued | 2023-08 | |
| dc.description.abstract | The present study investigated the effect of different probiotics on water quality parameters, floc formation, proximate composition, bacterial diversity, plankton diversity, growth parameters, haematological parameters, and histological parameters in a biofloc system. Treatment T1 includes (Nitrosomonas, Nitrobacter, Rhodococcus, Bacillus pumilus), and treatment T2 consist of (Bacillus subtilis, Bacillus megatherium, Bacillus licheniforms) and treatment T3 consist of different strains of bacteria (Pediococcus, Lactobacillus, Enterococcus, Bacillus). The results showed significant improvements in water quality parameters in the probiotic-supplemented biofloc system compared to the control group. The mea temperature was 29°C in the culture provided a stable environment for the growth of common carp. Dissolved oxygen (4.91-5.48 mg/l), pH value (6.75-8.70), total dissolved solids (1102-1424 mg/l), conductivity (976.25-2054.67 μs/cm), ammonia (0-0.77 mg/l), nitrite (0-0.96 mg/l), nitrate (0-51 mg/l), alkalinity (44-139 mg/l), salinity (0.35-1.07 ppt), hardness (112-383 mg/l), calcium (42-357 mg/l), magnesium (5.01-19.60 mg/l), and chloride levels (0.10-0.58 ppt) were within suitable ranges for the growth and health of aquatic organisms. The proximate composition of the floc varied significantly among treatment groups. The highest levels of crude protein, crude lipid, and crude fiber were observed in treatment T2, followed by treatment T1 and then T3. The THB count was log 4.84 CFU/ml after one week and reached log 6.35 CFU/ml by the end of the experiment. Total coliform count consistently decreased and aerobic bacteria increase in the probiotic treatment. Bacterial sequencing revealed that 30% of the isolated bacteria were identified as Bacillus. Microbacterium (20%) and Stenotrophomonas (20%) were other bacterial genera that were abundant in all isolated strains. The remaining 30% of isolated strains belonged to the genera Shewanella, Kocuria, and Ensifer. Treatment T2 had the highest plankton density (2.06±0.17×105 cells/l), followed by T1 (1.74±0.16×105 cells/L) and T3 (1.70±0.16×105 cells/L). The growth parameters of common carp, including weight gain (48.90±4.48 g), length gain (13.96±0.41 cm), average daily weight gain (0.38±0.019 g), and specific growth rate (1.74±0.04%), were significantly higher in treatment T2 compared to the other treatments and control group. However, the feed conversion ratio was significantly lower in treatment T2 (1.56±0.21) compared to the control group (1.77±0.21). At the end of the experiment, a survival rate of 96.66% was recorded in the treatment group, while the control group exhibited a survival rate of 90%. Hematological responses were monitored in probiotic treatments exposed fish and compared with the control. Among the different treatment group, significantly higher RBC count, WBC count, haemoglobin content and Packed cell volume was recorded in treatment T2 as compared to control group. Histomorphology of intestine of common carp revealed that villus width and villus height increased significantly (p<0.01) in the treatments groups as compared to control group. Biochemical parameters like AST, ALT activity differed significantly, with the highest activity noticed in the control group. | |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810211400 | |
| dc.language.iso | English | |
| dc.pages | 94+xv | |
| dc.publisher | CCSHAU, Hisar | |
| dc.sub | Zoology | |
| dc.theme | Effect of probiotics on the growth performance of Cyprinus carpio L. culture under biofloc technology/system | |
| dc.these.type | Ph.D | |
| dc.title | Effect of probiotics on the growth performance of Cyprinus carpio L. culture under biofloc technology/system | |
| dc.type | Thesis |