SHELF LIFE OF Trichoderma asperellum in DIFFERENT OIL FORMULATIONS.
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Date
2019-07-23
Authors
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Publisher
Dr. Punjabrao Deshmukh Krishi Vidyapeeth, Akola, Maharashtra.
Abstract
An investigation entitled “Shelf life of Trichoderma asperellum in different oil formulations” was carried out during 2018-2019. paraffin oil, soybean oil, groundnut oil, potato dextrose broth and talc was extensively used as carrier for Trichoderma asperellum. As compared to solid based carrier material the survival of Trichoderma asperellum in liquid based formulations is quite high and have the ability to limit the heat transfer high water holding capacity and maintain water around the cells for their metabolism. Shelf life studies clearly indicated significant differences at all the intervals. Population density of T. asperellum in the paraffin oil was 28.00 x 108 CFU/ml at 30 days whereas the population density of T. asperellum in the 180 days was 18.33 x 108 CFU/ml. It was found significantly superior over all other treatment. The per cent spore germination in formulation containing paraffin oil is 59.18 per cent. Maximum colonies was observed in pH range of 5.2-5.7 in paraffin oil treatment (T1) when the effect of liquid formulation on pH of Trichoderma asperellum was studied. It is significantly superior over the other treatment which we have under study. This ph range were found to be optimal for antagonistic potential of T. asperellum against Fusarium oxysporum f. sp. ciceri, Rhizoctonia bataticola and Sclerotium rolfsii.
The effect on percent growth inhibition on Fusarium oxysporum f. sp. ciceri is 74.44 per cent, on Rhizoctonia bataticola is 81.85 per cent and Sclerotium rolfsii is 72.6 per cent. It was recorded maximum in formulation containing paraffin oil it was found significantly superior over all the treatments. The effect of liquid formulations on spore germination of T. asperellum was also recorded the maximum spore germination are found in formulation containing paraffin oil after 180 days of storage.
The effect of Trichoderma formulation on seed germination, root length, shoot length, SVI of bengal gram, when seed inoculated with Fusarium oxysporum, Rhizoctonia bataticola, Sclerotium rolfsii by using paper towel method maximum germination percentage was 87.33, 74.33, 73.00 percent respectively in T1 (paraffin oil) . However root and shoot length was maximum in T1 (21.66 cm and 31.33 cm), (19.66 cm and 30.66 cm), (18.66 cm and 30.00 cm) respectively. The maximum seedling vigour index was found in paraffin oil seed treatment.
Description
Paraffin oil, soybean oil, groundnut oil, PD broth and talc are extensively used as carrier for Trichoderma asperellum. The survival of Trichoderma asperellum in liquid based formulations is quite high and as compared to solid based carrier material it has also advantage for improving survival and a good protection of Trichoderma asperellum. The observation on shelf life of Trichoderma asperellum with paraffin oil, per cent spore germination, percent inhibition and pH of oil formulations were recorded. Similarly different carriers were also tested for shelf life of Trichoderma asperellum.
From the recent study the shelf life of Trichoderma asperellum is maximum in treatment containing paraffin oil followed by soybean oil and it also gives maximum per cent growth inhibition in Fusarium oxysporum f. sp. ciceri, Rhizoctonia bataticola and Sclerotium rolfsii.
Keywords
Liquid formulation, Solid formulation, Solutions, Trichoderma harzianum, Trichoderma viride.
Citation
MANE, KU. KAJAL KISAN. (2019). Shelf life of Trichoderma asperellum in different oil formulations. Department of plant pathology, Nagpur. Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola. M. Sc. 2019. Print. xii, 59p. (unpublished).