SILKWORM CHITOSAN AND PUPAL OIL - THEIR EXTRACTION AND PHYSICOCHEMICAL PROPERTIES

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dc.contributor.advisorAMARNATHA N
dc.contributor.authorDUKARE PRADIP GULABRAO
dc.date.accessioned2024-03-13T10:37:12Z
dc.date.available2024-03-13T10:37:12Z
dc.date.issued2024-02-15
dc.description.abstractStudies on extraction of silkworm chitosan and pupal oil and their physico-chemical properties were conducted at the Department of Sericulture, UAS, GKVK, Bengaluru. The results revealed that, oil content in silkworm pupae ranged from 26 to 28 per cent on dry weight basis. Notably, female pupae had a higher oil content (27.99%) compared to males (27.03%). The iodine and saponification values of pupal oil was better compared to groundnut and sunflower oils. Similarly, the acid value (1.570 mg KOH/g oil) and peroxide value (2.453 ppm) were found to be lower than three in pupal oil samples which revealed that, pupal oil is good for edible purposes. The chitin and chitosan were significantly higher in male pupae (3.242% and 2.430%, respectively) than females (3.013% and 2.345%, respectively). Among the hybrids, chitosan content was higher in the cross breed (2.526%) than bivoltine hybrid (2.308%). Further, chitin and chitosan percentages were analysed in larval instars, moult exuviae and pupal exuviae. Notably, the bivoltine larval exuviae (after spinning) exhibited a significant increase in chitin and chitosan content (26.02% and 19.37%, respectively) compared to cross breed larval exuviae (25.89% and 19.28%, respectively). The moisture content of larval chitosan ranged between 7.00-8.00 per cent. However, pupal chitosan showed 7.20-7.40 per cent moisture. Viscosity of pupal chitosan was 44-46 cP, while larval/exuvia chitosan ranged from 48-58 cP. Extracted chitosan had ash content below 1 per cent, with pupal chitosan ranging from 0.30-0.38 per cent and larval/exuvia chitosan from 0.50-0.80 per cent. The degree of deacetylation and solubility ranged between 85-96 per cent and 92-99 per cent, respectively. The chitosan extracted from silkworm pupae shows better physico-chemical parameters like solubility (99%), degree of deacetylation (>85%) and ash content (<1%) which indicates high-grade chitosan and which can be utilized as an alternative source of chitosan extraction.
dc.identifier.otherTh-14035
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810207471
dc.keywordsSILKWORM CHITOSAN AND PUPAL OIL
dc.keywordsPHYSICO-CHEMICAL PROPERTIES
dc.language.isoEnglish
dc.pages109
dc.publisherUNIVERSITY OF AGRICULTURAL SCINCES BANGALORE
dc.subSericulture
dc.subjectSILKWORM CHITOSAN AND PUPAL OIL
dc.subjectPHYSICO-CHEMICAL PROPERTIES
dc.themeSILKWORM CHITOSAN AND PUPAL OIL - THEIR EXTRACTION AND PHYSICOCHEMICAL PROPERTIES
dc.these.typeM.Sc
dc.titleSILKWORM CHITOSAN AND PUPAL OIL - THEIR EXTRACTION AND PHYSICOCHEMICAL PROPERTIES
dc.typeThesis
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