Morphological- and biochemical-characterisation and molecular marker analysis of locally adapted maize inbreds for kernel -carotenoids and -tocopherols.
Loading...
Date
2019-07-23
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
CSKHPKV, Palampur
Abstract
The present investigation entitled “Morphological- and - biochemical- characterization and molecular marker analysis of locally adapted maize inbreds for kernel-carotenoids and- tocopherols”had been undertaken to carry out morphological and biochemical grouping of the selected panel if twenty four inbred lines planted at four location viz., Bajaura, Palampur, Kangra and IARI,Delhi. Biochemical analysis performed by UPLC grouped the inbred lines into genotypes with high level of provitamin-A and tocopherols. The only line viz., BAJIM-BIO-8 among the selected lines to show favorable allele for crtRB1 gene, apart from checks viz., PMI- PV1 and PMI-PV2. SSR analysis performed using gene-specific marker was able to locate the favorable allele for both carotenoids and tocopherols. Among tocopherols, α-tocopherol possesses highest vitamin E activity. Six lines viz., BAJIM-08-26, LM16, LM17, BAJIM-BIO-3, BAJIM- BIO-5 and BAJIM-BIO-6 were proven to be the lines with high mean α-tocopherol concentration suggesting presence of favorable allele if ZmVTE4 gene. Molecular diversity analysis using 75 SSR markers spanning the entire genome revealed a total of 195 alleles with mean major allele frequency of 0.65, mean PIC value of 0.38, mean heterozygosity of 0.04, 16 unique alleles and 18 rare alleles. Dissimilarity using Jaccard’s coefficient was calculated and 31 diverse cross combinations were revealed. Cluster diagram grouped the lines into three major clusters. Principle coordinate analysis was further able to plot the inbred lines based on their genetic distances into four quadrangles with similar genotypes lying in the same quadrangle. The lines which were identified for carotenoids and tocopherols can be used as donor for development of biofortified hybrids with high grain yield.
Description
Keywords
null