Molecular analysis of antagonistic effect of Trichoderma to Fusarium in Grand Nain Banana plant
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Date
2019
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Birsa Agricultural University, Ranchi
Abstract
Production of banana is highly affected due to Fusarium Wilt also called Panama disease, caused
by Fusarium oxysporum all around the world, leading to higher economic loss. The disease now
is widespread and destructive in almost all the banana-growing states in India,
Trichoderma harzianum was found to be most effective in inhibiting the growth of Fusarium
oxysporum . plants treated with Trchoderma harzianum with Fusarium oxysporum at different
interval 3 and 5 days ( Treated, Recovery + Fusarium). The result revealed that Trichoderma
harzianum is effective in antagonizing Fusarium spp. by mycoparasitic activity and also by
competing for rhizosphere colonization and nutrient. Plants treated with Trichoderma have some
altered metabolic activities. Thus, any changes was analyzed by visual observation and marker
analysis. The OPA series of primer was found to amplify the DNA sample of Trichoderma
harzianum, Fusarium oxysporum and Banana. In standardization process the best temperature
(annealing and extension) was selected with the better primers which amplified all the DNA
samples. Distinct bands were observed. OPA 02 at 72⁰C extension 55⁰C annealing temperature
showed some bands but the distinct bands were not observed. However, further refinement of the
protocol is needed.
Description
Molecular analysis of antagonistic effect of
Trichoderma to Fusarium in Grand Nain
Banana plant