Chemistry of sesquiterpenoids from essential oil of Cyperus scariosus and their antioxidant and antifungal potential
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Date
2018
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Punjab Agricultural University, Ludhiana
Abstract
The present investigation deals with Chemistry of sesquiterpenoids from essential oil of
Cyperus scariosus and their antioxidant and antifungal potential. The roots of C.
scariosus were grounded and subjected to hydro-distillation using Clevenger apparatus to
extract the essential oil. The essential oil was analysed by gas chromatography-mass
spectrometry. A total of 30 compounds, accounting for 93.30 % of the total essential oil, were
identified. Longiverbenone, nootkatone, caryophyllene oxide and cyperene were found to be
the major compounds present. The C. scariosus roots were also extracted by Soxhlet extractor
using three different solvent i.e. hexane, dichloromethane and methanol. Total of 55, 67 and
25 compounds were present in hexane, dichloromethane and methanol extracts respectively.
Cyperene, isolated from essential oil was chemically derivatized into cyperotundone,
cyperene alcohol, cyperenol, cyperenal and cyperene epoxide. The structures of the
compounds were elucidated on the basis of FTIR, 1H NMR and 13C NMR spectroscopy. C.
scariosus essential oil, various extracts, isolated and derivatized compounds were tested for
antifungal and antioxidant activities at different concentrations viz.0.05, 0.1, 0.25, 0.5 and 1.0
mg/mL respectively. All the extracts and essential oil showed significant antifungal activity.
Essential oil was most active as compared to other tested components against all plant
pathogens. The antioxidant activity of C. scariosus essential oil, various extracts, isolated and
derivatized compounds was carried out in vitro using involving 2,2-diphenyl-1-picrylhydrazyl
(DPPH), hydroxyl radical scavenging potential, inhibition of nitric oxide radical generation
and ferric reducing antioxidant power assay. Methanol extract of C. scariosus showed highest
antioxidant activity in all the methods tested as compared to essential oil, hexane and
dichloromethane extracts. Cyperenol showed highest antioxidant activity as compared to
isolated and derivatized compounds.
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