Analysis of detoxification and phenolic metabolism mediated through nitric oxide and hydrogen peroxide under ABA and water stress in wheat
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Date
2016
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Punjab Agricultural University, Ludhiana
Abstract
Two wheat cultivars PBW644 (drought tolerant) and PBW343 (drought sensitive) were compared
under PEG/ABA for ROS/nitric oxide (NO)-regulation of antioxidant metabolism. Endogenous
ROS/NO under ABA/PEG increased germination and growth, such effect was higher in PBW644.
In PBW343, reduced growth under PEG was improved by exogenous ROS/NO. ABA/PEGupregulation
of GPX and GST involved ROS/NO signalling which was similar in both cultivars
under ABA but higher in PBW644 under PEG. PEG increase of acid protease was higher in
PBW343 while of alkaline protease was higher in PBW644. ABA/PEG-upregulation of proteases
involved ROS/NO-signalling. Redox state of glutathione under PEG/ABA involved ROS/NO
signalling which was induced in PBW343 while in PBW644, it was induced when exogenous
ROS/NO applied under ABA (high stress level). Increase of ROS/NO under ABA/PEG was
signal, it was higher in PBW644. Such signals were increased by ROS/NO-signalling. Moreover,
ROS/NO-signalling also decreased ROS under ABA/PEG. Oxidative toxicity was lesser in
PBW644 under PEG, was reduced by ROS/NO signals in both cultivars. However, exogenous
ROS/NO under ABA could also increase oxidative toxicity. Under PEG, PBW644 showed higher
upregulation of cell wall peroxidase (CW-POX) while lesser soluble peroxidase (S-POX).
However, under ABA, it showed higher levels of both CW-POX and S-POX. ROS/NO signals
under PEG/ABA contributed to increase both types of POX in both cultivars. PEG increase of
phenolics and ABA/PEG increase of lignin was higher in PBW644. Under PEG/ABA, ROS/NOsignalling
increased phenolics and decreased lignin in PBW343 while in PBW644, it increased
both in shoot while decreased both in root. PEG increased flavonoids. Under PEG/ABA, ROS/NO
signals may increase flavonoids.
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Keywords
Wheat, antioxidant, nitric oxide, reactive oxygen species, abscisic acid, water stress