Fine mapping of known QTL region for yellow mosaic disease resistance in soybean (Glycine max L. Merrill)
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Date
2021
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Punjab Agricultural University, Ludhiana
Abstract
A set of one hundred and forty-eight RILs were derived from the cross of SL958 (R) × AGS456 (S) with an aim to fine map the known QTL region for yellow mosaic disease resistance and to evaluate for grain yield and component traits were grown in the field during kharif, 2020. The experimental material was sown in randomised block design in three replications. Observations on yield and its component characters were recorded along with the YMD score. All the 148 RILs segregated in all YMD infection categories. Analysis of variance showed the presence of significant variation in RILs for all the characters recorded. Seed yield was found to have highest GCV, PCV and genetic advance. Plant height was found to be highly heritable. Seed yield recorded significant and positive correlation with number of seeds per pod, number of pods per plant, harvest index, 100-seed weight, days to 50% flowering and plant height. The results of path coefficient analysis indicated that number of pods per plant and number of seeds per pod have highest and positive direct effect on seed yield. For fine mapping of known QTL region, a total of 187 SSR markers within the known QTL region were used for parental polymorphic studies and 43 markers were found to be polymorphic. Bulk segregant analysis was conducted with 43 polymorphic markers. Out of 43 markers, 20 markers showed polymorphic on both the resistant and susceptible bulks. One major QTL explaining 58% variation for YMD resistance was observed between the markers BARCSOYSSR_06_0718 and BARCSOYSSR_06_0684 (located at a distance of 2 cM on either sides of QTL region). Gene annotation revealed that begomovirus resistance associated genes i.e; SGS3 was present in the identified QTL. The SGS3 gene along with another begomovirus resistance gene i.e; RdRp1 were amplified on genomic DNA of AGS456 and SL958. The role of differential expression of these genes in compatible and incompatible soybean-MYMV interaction is further needed to be studied. The identified markers in chromosome 6 can facilitate marker assisted selection, provide a base for cloning of YMD resistance in soybean and also help in introgressing of YMD resistance into susceptible but well adapted and popular soybean varieties.
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Meghana D P (2021) Fine mapping of known QTL region for yellow mosaic disease resistance in soybean (Glycine max L. Merrill) (Unpublished M.Sc. thesis). Punjab Agricultural University, Ludhiana, Punjab, India.