BIOCONTROL EFFICACY OF Trichoderma MUTANTS AGAINST Fusarium oxysporum f. sp. ciceri CAUSING CHICKPEA WILT.
| dc.contributor.advisor | Ingle, Dr. S. T. | |
| dc.contributor.author | TEKALE, SHUBHAM SAHEBRAO. | |
| dc.date.accessioned | 2021-05-30T14:27:50Z | |
| dc.date.available | 2021-05-30T14:27:50Z | |
| dc.date.issued | 2021-03-05 | |
| dc.description | The present investigation was carried out at Post Graduate Institute, Department of Plant Pathology, Dr. PDKV, Akola during academic year 2018-2020. Here under aseptic condition percent growth inhibition i.e. efficacy was checked along with estimation of chitinase enzyme. Effective mutants trial was carried out in pot culture to study disease incidence and inoculum propagule density per gram of soil. | en_US |
| dc.description.abstract | Chickpea (Cicer arietinum L.), one of the major pulses cultivated and consumed in India, is also known as Bengal gram. Chickpea crop suffers from a number of seed borne and soil borne diseases. Among these wilts caused by Fusarium oxysporum f. sp. ciceri reported to be the most widespread disease and causes considerable yield losses. Considering increasing intensity and losses caused due to this disease, studies were carried out to assess the biocontrol efficacy of Trichoderma mutants against Fusarium oxysporum f. sp. ciceri causing chick pea wilt. Diseased samples showing typical symptoms were collected from field and resorted for isolation. In the pathogenicity test, F. oxysporum f. sp. ciceri found pathogenic to chickpea causing wilt disease. It showed yellowing, drooping and drying of leaves followed by drying of branches or entire plant. Trichoderma is a promising antagonistic organism for the biological control of plant pathogenic fungi. While the use of Trichoderma as bio-control agent against target pathogens, it is important to consider its efficiency. The present investigation aimed to use Trichoderma asperellum mutants prepared by inducing mutagenesis through Gamma ray’s technique for genetic improvement of the Trichoderma asperellum to enhance its biocontrol abilities against Fusarium oxysporum f. sp. ciceri. The pure culture of Trichoderma asperellum mutants were obtained from Department of Plant Pathology and code was given to each mutant for maintaining their identity. Then antagonistic potential of Trichoderma asperellum mutants were tested against Fusarium oxysporum f. sp. ciceri by dual culture technique. Among the mutants, TaMG-3 (T3) recorded highest growth inhibition i.e., 72.85% which was at par with TaMG-7 (T7) and TaMG-6 (T6) i.e., 72.47% and 70.78% respectively. Mother culture TaMC (T11) recorded 62.17% growth inhibition of Fusarium oxysporum f. sp. ciceri. The mutants were assayed for estimation of chitinase enzyme and the mutants TaMG-3 (T3) found to possess highest chitinase enzyme units/mg of protein i.e., 0.68. TaMG-4 (T4) and TaMG-7 (T7) were the next best and at par mutants which recorded 0.67 chitinase enzyme units/mg of protein respectively. Mother culture TaMC (T11) contained only 0.40 chitinase enzyme units/mg of protein. Mutants showed maximum efficacy in dual culture and the highest chitinase containing mutants were also evaluated against chick pea wilt in pot culture. The percent wilt incidence and Propagule density of pathogen was also recorded at 30, 45 and 60DAS. Initial Propagule density per gram of soil was also recorded. Among the Trichoderma asperellum mutants, T1 (TaMG-3) was found most effective for controlling chickpea wilt caused Fusarium oxysporum f. sp. ciceri at 30, 45 and 60 DAS. Propagule density of pathogen were drastically reduced in all treatments however T1 (TaMG-3) was also found effective for reducing the propagule density of pathogen at 30, 45 and 60 DAS. | en_US |
| dc.identifier.citation | TEKALE, SHUBHAM SAHEBRAO. (2020). Biocontrol efficacy of Trichoderma mutants against Fusarium oxysporum f. sp. ciceri causing chickpea wilt. Department of Plant Pathology, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola. M. Sc. 2020. xiv, 72p. (Unpublished). | en_US |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810169414 | |
| dc.keywords | Plant Pathology, Trichoderma mutants, Fusarium oxysporum f. sp. ciceri, Per cent growth Inhibition, Chitinase enzyme, Pathogenicity test, Per cent Disease incidence, Propagule density. | en_US |
| dc.language.iso | English | en_US |
| dc.pages | xiv, 72p. | en_US |
| dc.publisher | Dr. Punjabrao Deshmukh Krishi Vidyapeeth, Akola, Maharashtra. | en_US |
| dc.research.problem | In the present research the researcher has tried to study the efficacy of Trichoderma mutants along with chitinase enzyme. | en_US |
| dc.sub | Plant Pathology | en_US |
| dc.theme | The present research deals with the efficacy of Trichoderma mutants which was tested against Fusarium oxysporum f. sp. ciceri along with Chitinase enzyme estimation, Pathogenicity test, Per cent Disease Incidence and Inoculum Density per gram of soil. | en_US |
| dc.these.type | M.Sc | en_US |
| dc.title | BIOCONTROL EFFICACY OF Trichoderma MUTANTS AGAINST Fusarium oxysporum f. sp. ciceri CAUSING CHICKPEA WILT. | en_US |
| dc.type | Thesis | en_US |