Studies on Physiological, Biochemical and Molecular changes associated with seed priming in Maize hybrids (Zea mays L.)
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Date
2013-03-26
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University of Agricultural Sciences, GKVK
Abstract
The present study was conducted to standardize the hydro-priming
protocol in two maize hybrids viz., Hema and Nithyashree to know the
physiological, biochemical and molecular changes associated with
priming in maize seeds. The crop specific efficacy of different
combinations of temperature and duration of soaking were standardized
based on the performance of seed quality attributes and the best priming
temperature was 25±10C, optimum duration of soaking was 24h. All the
physiological attributes such as first count germination, final count
germination, T50, BRI, MSL, MSDW, SVI-I and SVI- II were significantly
higher (82%, 90%, 2.64 days, 0.409, 32.83cm, 6.05mg, 2990, 549, 81%
and 81%, 90%, 2.39 days, 0.406, 32.08 cm, 5.77mg, 2925, 526, 79% in
Hema and Nithyashree respectively). Biochemical attributes such as
TDH, TSP and DNA content were significantly higher (2.293, 282.58
μg/g, 44.45 μg/g and 2.074, 268.29 μg/g, 42.30 μg/g respectively in
Hema and Nithyashree respectively). Whereas, EC and TSS activity were
significantly lower (209.9 μSm-1, 145.37 μg/ml, and 200.52μSm-1,
142.17 μg/ml) in both hybrids.
The ability to distinguish and identify the hybrids is fundamental
for the operational aspects in the seed trade. SSR molecular markers
were employed to distinguish the hybrids and their parental lines. Among
48 SSR markers Only five viz., bnlg 1018, p-phi 435417, Umc 1388,
UMC 2362 and Phi 001 were found to be polymorphic between hybrids
and its parental lines. Based on the complementary banding pattern
between the hybrids and their parents the SSR marker bnlg 1018, P-phl
435417, Umc 1388 were identified as specific markers to distinguish F1
maize hybrids. The SSR primers UMC 2362 and Phi 001 were identified
as more useful primers which can differentiate the Hema hybrid from
Nithyashree and also from other hybrids. The chemical method of
varietal identification needs to be standardized and can be successfully
employed with other reliable methods of varietal identification.
Description
Keywords
sowing, hybrids, germinability, maize, soaking, developmental stages, rice, seed treatment, dna, seed vigour