Synthesis, Characterization and Safety Assessment of Nano Selenium and Organic Selenium for Incorporation in Lamb Feed
Loading...
Date
2022-03
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Background: Selenium is an important trace mineral required by the animals. It is an integral part of antioxidant system protecting
the body against free radical injury. Nano particles attract a widespread attention due to its high bioavailability and efficacy. The
current study was aimed to synthesize, characterize nano selenium and evaluate the cytotoxic effect of nano selenium and organic
selenium (selenocysteine) under in vitro condition in vero cell line.
Methods: Nano selenium was synthesized by wet chemical method by using sodium selenite, selenium powder, ascorbic acid and
sodium hydroxide at laboratory level. In this study particle size, shape, zeta potential and selenium content were characterized by
using particle size analyser (PSA), transmission electron microscope (TEM) and inductively coupled plasma mass spectrometry
(ICP-MS). The toxicity was analysed by MTT assay against vero cell line.
Result: The result revealed that selenium nano particles were spherical in shape, nano in size (less than 50 nm) and pure in nature.
The nano selenium and organic selenium (selenocysteine) effectively inhibited the growth of vero cells in a dose dependent manner.
Description
TNV_AJDFR_2022_41(1)77-82
Keywords
Veterinary Science