MARKER ASSISTED SELECTION FOR MAPPING POPULATION OF F5 GENERATION AGAINST CHICKPEA WILT
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Date
2020-12-18
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Dr. Punjabrao Deshmukh Krishi Vidyapeeth, Akola, Maharashtra.
Abstract
Chickpea (Cicer arietinum L.) is one of the most important legume crop called as “poor man’s meat” and “rich man’s vegetable”. It is a self pollinating, diploid (2n = 16) with small genome size of about 738 Mbp. The crop is vulnerable to many diseases viz. Fusarium wilt and Ascochyta blight etc.
Fusarium wilt of chickpea is caused by Fusarium oxysporum f. sp. ciceri. The pathogen is soil borne and present internally in seed. It was carried in hilum region of seed. Fusarium wilt of chickpea produces three types of asexual spores i.e. Microconidias, Macroconidias and Chlamydospores. The microconidias (2.5–4.5 μm × 5–11 μm) are oval or cylindrical, straight or curved. Macroconidias (3.5–4.5 μm × 25–65 μm) are produced more sparsely than microconidias and usually they are three to five septate or fusoid. Chlamydospores are thick walled vegetative cell formed singly, in pairs or in chains. Characteristic symptom of wilted plant is the stem of plant when split opened the blockage of xylem vessels can be seen. Yield losses vary between 10% to 100% depending on the varietal susceptibility and agroclimatic conditions.
Now a days, application of chemical pesticides is limited because of their environmental pollutions and health hazard. Therefore, disease can be managed by the use of disease resistant varieties. Hence, the present investigation was carried out to select the resistant of amongst the mapping population through marker assisted selection.
The investigation was carried out in the laboratory of Department of Plant Pathology, PGI, Dr. PDKV, Akola. The 40 chickpea genotypes were selected for screening from four mapping population of different crosses JG 62 (susceptible) X JAKI 9218 (resistant), PG 04305 (resistant) X JG 62 (susceptible), PG 07101 (resistant) X JG 62 (susceptible) and JG 62 (susceptible) X AKG 1001 (resistant). 10 genotypes from each population were selected.
Marker assisted characterization of 40 chickpea genotypes differing for Fusarium wilt reaction was carried out using two molecular markers reported by earlier workers linked to disease resistant/susceptibility. In the present study, Allele Specific Associated Primer (ASAP) marker (namely, CS-27F/R700) linked to susceptibility and two Sequence Tagged Microsatellite site (STMS) marker (TA-59258 and TA-96275) linked to resistance allele were validated.
The molecular markers ASAP (CS27F/R700), and STMS (TA59258 and TA96275) amplified against 40 genotypes of chickpea F5 generation.
The results indicate that ASAP (CS 27F/CS 27R) marker linked to susceptibility alleles and susceptibility is dominant over resistance. ASAP marker, (CS27F/R) screened on the 40 genotypes and gave no amplified product of 700 bp. in all 40 genotypes of cross I (JG 62 X JAKI 9218), in cross VI (PG 04305 X JG 62), in cross VII (PG 07101 X JG 62) and in cross VIII (JG 62 X AKG 1001). The absence of marker indicated resistance (h1h1h2h2) in all 40 genotypes.
STMS marker, TA-59 and TA-96 linked to resistance and produce amplification in resistant genotypes. The amplification sizes of TA-59 and TA-96 as characterized in cicer sp. were 258 and 275 bp, respectively. Amplification was observed in all 40 genotype of cross I (JG 62 X JAKI 9218), in cross VI (PG 04305 X JG 62), in cross VII (PG 07101 X JG 62) and in cross VIII (JG 62 X AKG 1001). Presence of marker indicated resistance in all 40 genotypes.
Description
The present investigation was carried out at Department of Plant Pathology, Dr. PDKV, Akola during academic year 2018-2020. The mapping population generated by crossing of JG 62 (susceptible) X JAKI 9218 (resistant), PG 04305 (resistant) X JG 62 (susceptible), PG 07101 (resistant) X JG 62 (susceptible) and JG 62 (susceptible) X AKG 1001 (resistant). The resistance and susceptibility of mapping population F5 generation was assayed by using Marker Assisted Selection through STMS (TA-59 and TA-96) and ASAP (CS-27F/R) markers.
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Citation
THAKARE, SIRISHA VIJAYRAO. (2020). Marker Assisted Selection For Mapping Population Of F5 Generation Against Chickpea Wilt, Department of Plant Pathology Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola. M. Sc. 2020. xii, 52p. (Unpublished).