Comparative evaluation of growth and physio-metabolic responses of Osteobrama belangeri (Valenciennes, 1844) in freshwater v/s saline water
Loading...
Files
Date
2022
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
West Bengal University of Animal & Fishery Sciences, Kolkata
Abstract
Salinity is one of the stressful environmental factors which affects life, metabolism, and distribution of aquatic animals. The present study on ՙՙComparative evaluation of growth and physio-metabolic responses of Osteobrama belangeri (Valenciennes, 1844) in freshwater v/s saline water ՚՚ was carried out to find the suitable salinity for growth performance and survival of pengba (Osteobrama belangeri) in inland saline water and to find the effects of different salinity concentrations on haematological, serum biochemical, and proximal parameters of this fish. In first phase of the experiment, 60 fingerlings of O.belangeri (11.78 ±0.057 g)were stocked in control and treatments (each having triplicate containing 10 sp each per replicate ) for 60 days to run a growth study by feeding with formulated (32 % protein ) feed @4% BW . Then in second phase, 18 days experimental study was run considering freshwater as control ( C ) and saline water as treatment (T) with gradually increasing salinities (2,4,6,8,10 and 12 ppt) at 72 hours interval in each salinity concentration. The result indicated that O. belangeri can tolerate the salinities up to 12 ppt. in inland saline water where as 100% mortality was observed in 12 ppt . MLS96h was calculated and LC 50 was determined using mortality data. Almost similar rate of growth and survival was found up to 8 ppt compared to control. Salinity more than 8 ppt appeared to be unsuitable for O. belangeri due to increased mortality and impaired growth. A few important physiological parameters i.e proximal value, behavioral changes were observed during 18 days of experimental phase . To determine haematological alterations, RBCs, haematocrit (HCT%), haemoglobin (Hb) , platelets count , WBC, MCH , MCHC , MCV values are determined and significant alterations ( p<0.05) found at different salinity concentration . To determine biochemical alterations, ALT (Alanine aminotransferase) or SGPT (Serum glutamate pyruvate transaminase), AST (Aspartate aminotransferase) or SGOT (serum glutamic oxalo acetic transaminase), SOD (superoxide dismutase) and Catalase (CAT) are examined collecting the blood serum. The increasing value of liver enzyme ALT and AST in blood indicated hepatic damages in higher salinities. However, all the biochemical parameters were found as almost normal up to 8 ppt compared with control. This evidence suggests that pengba can sustain and grow well below 8 ppt and can be a potential candidate for culture in coastal areas after heavy downpour when the salinities level falls to 8 ppt or lower.