EVALUATION OF SELECTED HERBS AS DIETARY SUPPLEMENTS ON GROWTH PERFORMANCE, SURVIVAL AND RESISTANCE AGAINST AEROMONAS HYDROPHILA INFECTION IN GENETICALLY IMPROVED FARMED TILAPIA (GIFT), OREOCHROMIS NILOTICUS FRY
Loading...
Files
Date
2020-11
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
JAU,JUANAGDH
Abstract
The objective of this research was to evaluate the effect of selected herbs on
growth performance and resistance of Genetically Improved Farmed Tilapia (GIFT),
fry challenged with Aeromonas hydrophila. In the current study for the improvement
of the growth performance and immunity different level of ashwagandha, shatavari,
turmeric, ginger, neem and tulsi were used in powder form as supplement in practical
diet. Sixty days experiments were conducted to understand the role of this selected
herbs administered through feed in growth, haematological response and disease
resistance of GIFT, fry against A. hydrophila.
The practical diet was formulated to contain different levels of mentioned
herbal powder (5, 10, 20 and 50g/kg diet) and control diet was free from the herbal
supplement. The diet was fed twice a day to fishes for 1 to 60 days. At the start of the
experiment, fish weighed in gram and measured length in centimetre was recorded. At
the end of feeding trials after 60 days, the experimental fishes were challenged with
0.1 ml of Aeromonas suspension @ 1 х 107 cells/ ml injected intramuscularly by
selecting 10 fishes from each treatment. The selected medicinal plants showed
increase trend of the Body length, body weight, SGR of fry of GIFT. At the end of
experiment the average initial and final length ranged between 2.2±0.12 and 8.5±0.12
cm and the average initial and final weight was ranged between 1.68±0.22 and
7.30±0.022 g respectively among all the treatments. The fish fed the Ashwagandha at
a concentration of 20 g/kg of feed showed 0.076±0.007, 0.077±0.005, 0.095±0.01,
0.096±0.02 and 0.090±0.04 in treatment AT0, AT1, AT2, AT3 and AT4 respectively.
The mean initial and final length of GIFT fry fed with different levels of shatavari
were ranged between 2.1±0.10 and 7.35±0.19 cm and the average initial and final
weight ranged between 1.64±0.013 and 7.29±0.031 gm respectively. The fish fed with
Shatavari at a concentration of 10 gm/kg of feed showed an enhanced specific growth
rate when compared with that of the control fish. It was recorded 0.074±0.002,
0.087±0.030, 0.098±0.050, 0.092±0.09 and 0.094±0.10 in treatment ST0, ST1, ST2,
ST3 and ST4 respectively. The treated fish fed the turmeric supplemets at the 20 g/kg
ii
level showed the significantly (P<0.05) greatest increases in length and weight. The
average initial and final length was ranged between 2.1±0.20 and 7.80±0.25 cm and
the mean initial and final weight was ranged between 1.67±0.01 and 7.51±0.88 g.
SGR observed 0.070±0.017, 0.074±0.002, 0.091±0.03, 0.095±0.02 and 0.090±0.04 in
treatment TRT0, TRT1, TRT2, TRT3 and TRT4 respectively when fish fed with
Turmeric at a concentration of 20 gm/kg. Fish fed 1% ginger supplemented diet had a
marked increase in growth performance parameters efficiently than control. The
average initial and final length was ranged between 2.1±0.20 and 6.40±0.18 cm and
weight was ranged between 1.67±0.0130 and 5.81±0.25g respectively. SGR were
recorded 0.065±0.011, 0.068±0.012, 0.079±0.09, 0.072±0.11 and 0.069±0.14 in
treatment GT0, GT1, GT2, GT3 and GT4 respectively. Initial mean length and weight
were recorded 2.1±0.14 and 1.67±0.0130 and final mean length and weight were
6.1±0.05 cm 5.59±0.25 gm respectively. The fish fed the neem at a concentration of
20 g/kg of feed showed an enhanced specific growth rate recorded 0.064±0.011,
0.064±0.012, 0.068±0.031, 0.072±0.021 and 0.065±0.012 in treatment NT0, NT1,
NT2, NT3 and NT4 respectively. The treated fish fed the tulsi supplemented diet at
the 20 g/kg level showed the significantly (P<0.05) greatest increases. The mean
initial and final length were ranged between 2.14±0.11 and 6.52±0.05 cm and weight
were between 1.59±0.14 and 6.38±0.40 respectively. The results demonstrate
enhanced haemoglobin content, total RBC counts, total WBC counts, super oxide
anion production and survival (P < 0.05) in treatments group compared with control
group. These results indicated that the addition of herbal powder to fish feed at the
rate of 10 and 20 g/kg of diet could enhance the growth, immune response and disease
resistance against A. hydrophila of GIFT fry during 60 days supplementation period.