Biochemical and molecular shifts in Solanum melongena alien introgression lines following JASSID, Amrasca bigutulla bigutulla (Ishida) infestation

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dc.contributor.advisorRimaljeet Kaur
dc.contributor.authorHanda, Sysha
dc.date.accessioned2023-12-11T12:59:52Z
dc.date.available2023-12-11T12:59:52Z
dc.date.issued2023
dc.description.abstractBrinjal jassid, Amrasca biguttula biguttula (Ishida) is emerging as devastating pest which significantly affects its yield and production. Varietal resistance could be an imperative and effective strategy to combat pest feeding. Vegetable specialist at PAU, Ludhiana developed S. melongena – S. insanum derived lines which displayed excellent variability to resistance against jassid infestation. Key resistance determinants at morphological, biochemical and transcriptional levels have not been explored so far. Therefore, we planned our research with the objective of determining jassid responsive defensive traits which encompasses phenotypic assessment, morphological characterization, biochemical alterations and gene expression profiling in S. melongena – S. insanum against jassid feeding. Phenotypic assessment of introgression lines (ILs) on the basis of jassid population per plant and pest percent intensity categorized genotypes into highly resistant, moderately resistant, moderately susceptible and highly susceptible genotypes. An increase in affected leaf area was determined with increase in the incidence of A. bigutulla bigutulla infestation. Genotypes with high trichome density and a short trichome length were resistant to pest attack. The increased expression of APX transcripts and ascorbate peroxidise (APX) activity along with a decrease in jassid count in resistant genotypes determined the pivotal role of APX in S. melongena genotypes against jassid attack. This candidate would be used in deployment of S. insanum resistance during brinjal breeding programs. On the contrary, susceptible genotypes showed higher expression of catalase (CAT) activity and CAT transcripts during jassid feeding. APX primarily decomposed H2O2 in resistant lines, whereas CAT primarily did so in susceptible genotypes. During high jassid infestation, H2O2 concentration rapidly increased in test genotypes. In comparison to resistant genotypes, H2O2 buildup was higher in susceptible genotypes. Increased total sugar and sucrose content in susceptible genotypes following jassid infestation suggests their contribution to the pest susceptibility. The genotypes with high jassid infestation displayed high oxidative stress (H2O2 and MDA), indicating that an increase in liposygenase (LOX) activity might cause high production of hydroperoxides, which in turn led to an increase in CAT, polyphenol oxidase (PPO), and phenolic accumulation. The association between JPP and H2O2, LOX, CAT, PPO, MDA, and total phenols was significant and positive. Thus, our findings suggest that susceptible genotypes experienced more oxidative stress than resistant genotypes.
dc.identifier.citationHanda, Sysha (2023). Biochemical and molecular shifts in Solanum melongena alien introgression lines following JASSID, Amrasca bigutulla bigutulla (Ishida) infestation (Unpublished M.Sc. thesis). Punjab Agricultural University, Ludhiana, Punjab, India.
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810202573
dc.keywordsJassid
dc.keywordsS. melongena
dc.keywordsS. insanum
dc.keywordstrichomes
dc.keywordsascorbate peroxidase
dc.keywordscatalase
dc.keywordstranscripts
dc.keywordsresistant
dc.keywordssusceptible
dc.keywordsgenotypes
dc.keywordsoxidative stress.
dc.language.isoEnglish
dc.pages140
dc.publisherPunjab Agricultural University
dc.research.problemBiochemical and molecular shifts in Solanum melongena alien introgression lines following JASSID, Amrasca bigutulla bigutulla (Ishida) infestation
dc.subBiochemistry
dc.themeBiochemical and molecular shifts in Solanum melongena alien introgression lines following JASSID, Amrasca bigutulla bigutulla (Ishida) infestation
dc.these.typeM.Sc
dc.titleBiochemical and molecular shifts in Solanum melongena alien introgression lines following JASSID, Amrasca bigutulla bigutulla (Ishida) infestation
dc.typeThesis
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