Putative bacterial consortium for mitigating wilt incidence in chickpea
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Date
2021
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Punjab Agricultural University, Ludhiana
Abstract
Despite the multifold importance, there was negligible increase in the chickpea production in the last decade due to Fusarium wilt caused by Fusarium oxysporum f. sp. ciceris (Foc). The present investigation focused on the development of bioinoculant formulations with dual plant growth promotion and biocontrol of Fusarium wilt in a sustainable manner. Mesorhizobium (10) and endophytic rhizobacteria (35) were screened for antagonism based on growth inhibition of Foc using dual culture and sealed plate assay. 93%, 76%, 64% and 53% isolates were active producers of protease, lipase, cellulase and chitinase, respectively. Biocompatible dual inoculants with no zone of inhibition were further screened for multifarious PGP traits viz. P-solubilization, production of IAA, gibberellic acid, ACC-deaminase, siderophore and biofilm. Efficacy of different liquid (LB, NB, LB+0.1%CMC, and NB+ 0.1%CMC) and carrier based (Talc and charcoal) formulations of dual inoculants was assessed up to 90 days. Highest viable count (8.668 log CFU ml-1) and optical density (0.8946 OD 600nm) was recorded in SG4 + FRW2 in NB + 0.1% CMC. Effect of bioinoculant formulations on growth, symbiotic traits, soil quality, yield and biocontrol of Fusarium sp. was recorded under wilt sick pot conditions in chickpea. Significant increase in growth and symbiotic traits was observed in SG4+ FRW2 in LB with 0.1% CMC as compared to inoculated control at 80DAS. An enhancement in peroxidase activity was observed in 0.1% CMC amended LB formulation LCRE9 + FRW2 (0.178 U min-1 mg-1 fresh weight of root) after the appearance of disease. Highest catalase
(11.31 U min-1 g-1 fresh weight of root), SOD (11.06 U min-1 g-1 fresh weight of root) and total phenol content (85.42 Tannic acid equivalents g-1 fresh weight of root) was recorded in SG 4 + FRW 2 in LB with 0.1% CMC with 4% superior wilt control over recommended charcoal formulation (RB1 + LGR33). Highest grain yield was recorded in bioinoculant formulation SG4 + FRW2 in LB amended with 0.1% CMC (1.91 g plant-1). SG4 + FRW2 and LCRE9 + FRW2 along with liquid formulations can be further developed to improve the chickpea productivity and reduce the disease severity of Fusarium oxysporum f. sp. ciceris in an ecofriendly manner.
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Jakilanki, Mounica (2021) Putative bacterial consortium for mitigating wilt incidence in chickpea (Unpublished M.Sc. thesis). Punjab Agricultural University, Ludhiana, Punjab, India.