Introgression profiling of F4 population derived from the cross of Zea mays × Teosinte spp. mexicana using SSR markers
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Date
2022
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Punjab Agricultural University, Ludhiana
Abstract
Teosinte is the wild ancestor of maize also known as God of grains. Teosinte populations could serve as a genetic bridge for transferring genes from one maize population to the next. The present investigation was carried out using 109 recombinant inbred lines (RILs) derived from the cross of Popcorn and Teosinte. RILs along with parental lines were grown in three replications and data was recorded for morphological traits like days to maturity, number of cobs, number of tillers, seed rows per cob and cob length. Analysis of variance showed significant variation among all traits. Highest positive correlation was observed between number of rows per cob and cob length. Genotyping was done using SSR markers from maize database spanning all the maize chromosomes. Out of 250 markers used, 35 were polymorphic. Introgression profiling of all the lines along with parents was done to analyze the introgressed regions of Popcorn and Teosinte in each RIL. Introgression profile of different lines were inferred from consensus of genotypic and morphological data which revealed that marker bnlg1297 was found to be common among the lines having more number of tillers and cobs. For lesser number of maturity days marker bnlg1131 was present in most of the lines. Therefore, these genomic regions might be associated with trait. Further, more number of markers can be used to identify the regions associated with different traits.
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Ashmita (2022). Introgression profiling of F4 population derived from the cross of Zea mays × Teosinte spp. mexicana using SSR markers (Unpublished M.Sc. thesis). Punjab Agricultural University, Ludhiana, Punjab, India.