EFFECTS OF EXOGENOUS FIBROLYTIC ENZYMES ON IN-VITRO DIGESTIBILITY OF DRY FORAGES 1741
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Date
2013-07
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JAU,JUNAGADH
Abstract
An experiment was conducted to evaluate effects of neutral
cellulase and fungal xylanase on in-vitro digestibility of dry jowar fodder and dry
pasture grass. The experimental study was carried out at Cattle Breeding Farm,
Junagadh Agricultural University, Junagadh. Neutral cellulase and fungal xylanase
enzymes were procured from Aumgene Biosciences Pvt. Ltd., Surat, Gujarat. Samples
of dry jowar fodder and pasture were collected. Samples were ground separately to
reduce particle size (0.5mm) and subjected to proximate and Van Soest analysis.
Different samples of dry jowar fodder and dry pasture grass were prepared according
to different concentrations of neutral cellulase and fungal xylanase. Rumen liquor for
in-vitro studies were collected in duplicate from two castrated bulls maintained on dry
jowar and pasture grass feeding with the help of stomach tube by applying suction.
During in vitro studies, trials were conducted to estimate various rumen fermentation
parameters such as total gas production, methane production along with in vitro DM
degradability, in vitro OM degradability, in vitro NDF degradability and in vitro ADF
degradability, ammonia nitrogen concentration, partitioning factor, short chain fatty
acid and microbial biomass production. These trials were conducted along with
respective blank in triplicate.
Statistically, significantly higher (P<0.05) total gas production was recorded
for neutral cellulase at 0.7 % and 0.8 %; for fungal xylanase at 0.5 %, 0.6 % and 0.8
% and for neutral cellulase + fungal xylanase (1:1) at 0.6% and 0.8 % from dry jowar
fodder. Statistically, significantly higher (P<0.05) total gas production was recorded
for neutral cellulase at 0.7 % and 0.9 %; for fungal xylanase at 0.8 % and for neutral
cellulase + fungal xylanase (1:1) at 0.7% and 0.8 % from dry pasture gi'ass.
Statistically, significantly higher (P<0.05) methane gas production was
recorded for neutral cellulase at 0.7 % and 0.9 %; for fungal xylanase at 0.8 % and for
neutral cellulase + fungal xylanase (1:1) at 0.6 % and 0.8 % from dry jo war fodder.
Statistically, significantly higher (P<0.05) methane gas production was recorded for
neutral cellulase at 0.8 %, for fungal xylanase at 0.7 % and for neutral cellulase +
fungal xylanase (1:1) at 0.8 % from dry pasture grass. Result data of ammonia
nitrogen did not show any statistically significant (P<0.05) effects for treated groups
of dry jowar fodder. Statistically, significantly higher (P<0.05) ammonia nitrogen was
recorded for neutral cellulase at 0.3 % to 1 % and for neutral cellulase + fungal
xylanase (1:1) at 0.7 % from dry pasture grass. Treated groups of dry pasture grass
with fungal xyanase did not show any statistically significant (P<0.05) results for
samples of dry pasture grass.
Statistically, significantly higher (P<0.05) TDMD (%) and TDOMR (mg) was
recorded for neutral cellulase at 0.8 %, for fungal xylanase at 0.6 % and for neutral
cellulase + fungal xylanase (1:1) at 0.7 % from dry jowar fodder. Statistically,
significantly higher (P<0.05) TDMD (%) and TDOMR (mg) was recorded for neutral
cellulase at 0.3% and 0.7 %, for fungal xylanase at 0.8 % and for neutral cellulase +
fungal xylanase (1:1) at 0.8 % from dry pasture grass.
Statistically, significantly higher (P<0.05) IVNDFD (%) was recorded for
neutral cellulase at 0.8 %, for fungal xylanase at 0.6 % and for neutral cellulase +
fungal xylanase (1:1) at 0.6 % from dry jowar fodder. Statistically, significantly
higher (P<0.05) IVADFD (%) was recorded for neutral cellulase at 0.7 % to 0.9 %,
for fungal xylanase at 0.6 % and for neutral cellulase + fungal xylanase (1:1) at 0.6 %
from dry jowar fodder. Statistically, significantly higher (P<0.05) IVNDFD (%) was
recorded for neutral cellulase at 0.3 % and 0.7 %, for fungal xylanase at 0.8 % and for
neutral cellulase + fungal xylanase (1:1) at 0.8 % from dry pasture grass. Statistically,
significantly higher (P<0.05) IVADFD (%) was recorded for neutral cellulase at 0.3
%, for fungal xylanase at 0.8 % and for neutral cellulase + fungal xylanase (1:1) at 0.8
% from dry pasture grass.
Statistically, significantly higher (P<0.05) MBP (mg) was recorded for neutral
cellulase at control group, for fungal xylanase at 0.6 % and for neutral cellulase +
fungal xylanase (1:1) at 0.9 % from dry jowar fodder. Statistically, significantly
higher (P<0.05) MBP (mg) was recorded for neutral cellulase at 0.3 %, for fungal
xylanase at 0.4 % and for neutral cellulase + fungal xylanase (1:1) at 0.8 % from dry
pasture gi'ass. Statistically, significantly higher (P<0.05) PF was recorded for neutral
cellulase at control gi'oup, for fungal xylanase at control and 0.01 % and for neutral
cellulase + fungal xylanase (1:1) at control gi'oup from dry jowar fodder. Statistically,
significantly higher (P<0.05) PF was recorded for neutral cellulase at 0.01 %, for
fungal xylanase at control group and for neutral cellulase + fungal xylanase (1:1) at
control group from di'y pasture gi'ass. Statistically, significantly highei (P<0.05)
SCFA (mmol) was recorded for neutral cellulase at 0.7 % and 0.8 %, for fungal
xylanase at control and 0.8 % and for neutral cellulase + fungal xylanase (1.1) at 0.6
% and 0.8 % from dry jowar fodder. Statistically, significantly higher (P<0.05) SCFA
(mmol) was recorded for neutral cellulase at 0.7 % and 0.9 %, for fungal xylanase at
0.8 % and for neutral cellulase + fungal xylanase (1:1) at 0.7 % and 0.8 % from dry
pasture grass.
Based upon in vitro studies with rumen fermentation parameters, 0.6 % to 0.8
% levels of mixture of neutral cellulase + fungal xylanase (1:1) can be the best
combination to increase degi'adability of dry jowar fodder and dry pasture grass.
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Keywords
ANIMAL NUTRITION