Study on natural allelic diversity in drought responsive Myb gene in germplasm of Eleusine coracana collected from different regions of Uttarakhand
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Date
2017-08
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G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand)
Abstract
Abiotic stresses like drought, salinity and cold cause huge losses to crop yield. MYB transcription factors have been found to be regulated by ABA signaling and modulate the expression of various downstream abiotic stress related genes. In the present study the effect of 11 day drought stress treatment on various genotypes of finger millet was evaluated at the vegetative stage. Drought stress was found to have an effect on various morpho-physiological and biochemical characters. DNA isolation of all the 12 genotypes was done and PCR amplification using gene specific primers was performed. The amplicons were sequenced and the sequences hence obtained were subjected to further analysis using various bioinformatics tools. Multiple Sequence Analysis results indicated that considerable nucleotide diversity for the drought responsive Myb gene is present in the studied finger millet germplasm. PRM 6107 was found to be the most drought tolerant genotype, amongst all the genotypes under study based on the various morpho-physiological and biochemical tests conducted. PCPGR 8115 was observed as the most sensitive genotype. Single nucleotide polymorphisms and insertions in the Myb allele of PRM-6107 were studied. PRM-6107 Myb allele was found to be highly similar to Saccharum barberi Myb 18 in terms of nucleotide sequence, protein sequence, protein structure and function and phylogeny. Saccharum barberi Myb 18 has a role in imparting dehydration and salinity tolerance. Hence, it was concluded from this study that finger millet genotype PRM-6107 harbours a superior drought tolerant allele for drought responsive Myb gene.
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