SPERM VIABILITY AND FERTILITY RATE OF KANGAYAM BULL SEMEN EXTENDED WITH DIFFERENT DILUENTS
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Date
2021
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Abstract
Awareness of artificial insemination (AI) in Kangayam and other native cows among farmers of Tamil Nadu state is increasing due to non-availability of pedigreed and suitable breeding bulls and the Jallikattu fest. Identification of suitable extender for cryopreservation of native bull semen is the need of hour in native animal breeding. Kangayam bull No NK 14 aged 5 years was utilized for the study. The semen was collected twice in a week and each time two ejaculates were collected. Then they were immediately transferred to semen processing laboratory and kept at 34° C in the water bath. The semen collected from the bull into the sterile disposable plastic graduated tubes was immediately subjected to macroscopic, microscopic and biochemical analysis. A two μl of neat semen sample was diluted with 400 μl of Tris solution. After dilution, two μl of sample was loaded in one chamber of prewarmed Leja slide and kept in microscopic stage of CASA and all the sperm kinetic characters were analyzed. Tris- egg - yolk glycerol (TEYG) (group I), soya lecithin based extender (group II) and liposome based extender (group III) were prepared on the day of semen collection and kept in water bath at 34° C for processing of semen. Initially the semen sample was diluted at 1:1 ratio with the corresponding extender (TEYG or soya lecithin based or liposome based extenders) according to the group. The 1:1 diluted semen samples from each group were kept at 22° C for 7 minutes in the laminar air flow. During this time the temperature of semen sample was reduced from 34° C to 22° C. After reaching 22° C, the 1:1 diluted semen samples from each group was finally extended with respective semen extender as per the dilution rate.