Protein profiling of Rhizobium isolates of Green Gram (Vigna radiata L.) collected from acidic soils of Jharkhand by employing 2-DE
Loading...
Date
2013
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Birsa Agricultural University, Kanke, Ranchi, Jharkhand
Abstract
Acidic soil in Jharkhand occupies approximately 90 percent area of the land. Soil acidity
affects many area of the world and limits legume productivity. Most leguminous plant
requires neutral or slightly acidic soil for growth, especially when depending on symbiotic
nitrogen fixation (correa and barneix, 1997). The major problems of legume growth in
strongly acidic soils are multiple nutrient disorders, including toxicities of H+, Al and Mn
and deficiencies of Ca, Mg, P and Mo (Jayasundara et al., 1998). However, few strain has
been indentified that can tolerate acidity and nodulate successfully. In current study
protein profiling of Rhizobium isolates collected from acidic soil was carried out. Protein
profiling involves comparison of two dimensional gels to understand various biological
processes by determining the presence or absence, up and down regulation, and
modification state of proteins in acidic stress. In the study we showed that the crop Vigna
radiata has different unique spots at various pH regimes. We analysed unique spots with
the help of Two Dimensional Gel Electrophoresis. Two Dimensional Polyacrylamide Gel
Electrophoresis of proteins is a robust and reproducible technique. It is the most widely
used separation tool in proteomics. Mung is a leguminous plant which shows symbiosis
relationship with the Rhizobium and produces nodules, within nodules, atmospheric
nitrogen converted into ammonia therefore proteomics is an ideal tool for the dissection of
plant microbe interactions. Proteomics provides a broad overview of the proteins produced
by both partners during their constant signal exchange. It also detects the signal
transduction pathways by following phosphorylation changes of proteins (Peck et al.,
2001). In our study Two-dimensional electrophoresis was done to analyze the various
proteins and their regulation under acidic condition. Rhizobial cell respond to acidity by
either up-regulation or down-regulation of genes that leads to the increased or decreased
translation of specific proteins. To study the stress proteins of Rhizobia by twodimensional
electrophoresis, protein profiles under different pH ranges were compared.
Through studying the proteome of Rhizobium in acidic soil condition, the response of the
isolates towards acidity of soil is being analyzed. At the molecular level, we find that the
two-dimensional gel analysis reveals a host of proteins which are found to be up-regulated
or down-regulated in response to different pH conditions. We hypothesize that adaptation
mechanism taking place in mung to resist and recover in response to abiotic stress such as
acidic soil.
Description
Protein profiling of Rhizobium isolates of Green
Gram (Vigna radiata L.) collected from acidic
soils of Jharkhand by employing 2-DE
Keywords
null