Molecular marker based assessment of population structure and dispersal potential of Cheilomenes sexmaculata (F.)

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Date
2023
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DIVISION OF ENTOMOLOGY ICAR-INDIAN AGRICULTURAL RESEARCH INSTITUTE NEW DELHI -110 012
Abstract
In India, farmers use various pesticides to manage the dreaded pests. The wide and indiscriminate use of pesticides causes destruction of beneficial organisms. Biological control considered as backbone of any IPM programme. The zigzag beetle, Cheilomenes sexmaculata which is most important and abundant of all ladybird beetles. C. sexmaculata were collected from five different zones contained from 25 subpopulations. We have identified 109,585 contigs, 304,784 SNPs, 46,583 INDELs. We observed 5 INDELs and 9 SSRs were polymorphic. We observed highly significant genetic diversity for West Zone followed by North Zone, Centre Zone, East Zone and South Zone respectively. Further analysis of genetic differentiation was highest gene flow (Nm) and FST was high in South Zone and East Population (FST=1) populations and Centre, North and West zones recorded FST=0. Mantel correlation revealed genetic distances decreases with the increasing geographical distance SNPs (Rxy= -0.184 and P= 0.07); INDELs (Rxy= -0.204 and P= 0.05) and SSRs (Rxy= -0.339 and P= 0.06). Released beetles for the most part left the released area (0m) within 24 hours. Beetles (39) from West zone reached border of 30m from the center of release at very early in the evening of 2nd day of release. Diffusion coefficient (D) was recorded the highest for beetles from North zone (1.99 m2 h-1) followed by West (1.95 m2 h-1), Centre (1.59 m2 h-1), South (1.41 m2 h-1) and East Zone (1.14 m2 h-1) populations. Relative departure rate (λ) was recorded the maximum for North zone (0.26 h-1), and the lowest in East Zone (0.07 h-1) population. Markers generated from this study will helps in analysing population structure and genetic diversity of same and related species of Coccinellidae and also the outcome of this study helps in selecting better strains for classical and augmentative biological control
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T-11079
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