Molecular marker based assessment of population structure and dispersal potential of Cheilomenes sexmaculata (F.)
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Date
2023
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DIVISION OF ENTOMOLOGY ICAR-INDIAN AGRICULTURAL RESEARCH INSTITUTE NEW DELHI -110 012
Abstract
In India, farmers use various pesticides to manage the dreaded pests. The wide and
indiscriminate use of pesticides causes destruction of beneficial organisms. Biological
control considered as backbone of any IPM programme. The zigzag beetle,
Cheilomenes sexmaculata which is most important and abundant of all ladybird beetles.
C. sexmaculata were collected from five different zones contained from 25
subpopulations. We have identified 109,585 contigs, 304,784 SNPs, 46,583 INDELs.
We observed 5 INDELs and 9 SSRs were polymorphic. We observed highly significant
genetic diversity for West Zone followed by North Zone, Centre Zone, East Zone and
South Zone respectively. Further analysis of genetic differentiation was highest gene
flow (Nm) and FST was high in South Zone and East Population (FST=1) populations and
Centre, North and West zones recorded FST=0. Mantel correlation revealed genetic
distances decreases with the increasing geographical distance SNPs (Rxy= -0.184 and
P= 0.07); INDELs (Rxy= -0.204 and P= 0.05) and SSRs (Rxy= -0.339 and P= 0.06).
Released beetles for the most part left the released area (0m) within 24 hours. Beetles
(39) from West zone reached border of 30m from the center of release at very early in
the evening of 2nd day of release. Diffusion coefficient (D) was recorded the highest for
beetles from North zone (1.99 m2 h-1) followed by West (1.95 m2 h-1), Centre (1.59 m2
h-1), South (1.41 m2 h-1) and East Zone (1.14 m2 h-1) populations. Relative departure
rate (λ) was recorded the maximum for North zone (0.26 h-1), and the lowest in East
Zone (0.07 h-1) population. Markers generated from this study will helps in analysing
population structure and genetic diversity of same and related species of Coccinellidae
and also the outcome of this study helps in selecting better strains for classical and
augmentative biological control
Description
T-11079