Postharvest studies in neelamari (Indigofera tinctoria L.)

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dc.contributor.advisorMeagle Joseph, P
dc.contributor.authorArogyamary Supritha, S
dc.contributor.authorKAU
dc.date.accessioned2020-01-14T08:12:58Z
dc.date.available2020-01-14T08:12:58Z
dc.date.issued2017
dc.descriptionPGen_US
dc.description.abstractNeelamari (Indigofera tinctoria L.) belonging to the family Fabaceae, is a widely used medicinal crop due to its properties. The main use of the Neelamari leaves is in the preparation of Neelibhrngadi Taila which is an Ayurvedic formulation known for its hair tonic property. The other important ingredients of the Neelibhrngadi Taila are Amla, Eclipta alba, Cardiospermum halicacabum, etc. The availability of Neelamari leaves restricted to a particular season does not always coincide with the season of Amla, which may result in a setback in the production of Neelibhrngadi Taila. In this context, the present investigation “Postharvest studies in Neelamari (Indigofera tinctoria L.)” was taken up to study the effect of harvesting stage and post-harvest storage on yield and quality of leaves. The effect of drying after subjecting to various pre- treatments on leaf and oil quality was also studied. Neelamari leaves were harvested at 70 days after transplanting (DAT), 115 DAT and 160 DAT. At each stage of harvest, the yield and quality of fresh leaves in terms of Relative Water Content (RWC), indican (Indoxyl β- D- glucoside) the glucoside responsible for the colour, total sugar and tannin were observed. The highest yield (48.05 g-1plant), was obtained when harvesting was done at 160 DAT and at this stage leaves had highest tannin (1.78mg 100g-1), total sugars (4.62%) and indican (1.23%) and it was significantly different from other treatments. At each harvesting stage, the leaves were stored for 4, 8, 24 and 48 hours and observed for its quality in comparison with fresh leaves. The freshly harvested leaves recorded higher value for indican (0.913%) but there was no significant difference in tannin (1.307mg 100g-1) and total sugars (4.70%). The interaction of cutting stage and storage showed significant effect on leaf quality as indicated by the highest indican content (1.24%) in fresh leaves harvested at 160 DAT and considering it as the best, was used for further studies. Three blanching methods including hot water, steam and microwave blanching were compared with unblanched control, followed by shade, cabinet and vacuum drying. The treated leaves were, analysed for indican content and found that, microwave blanching for 45 seconds followed by vacuum drying at 35±5°C had highest retention of indican (3.045%). Further Neelibhrngadi Taila was prepared using the decoctions of dried leaf samples, along with fresh Neelamari leaf juice considered as control treatment as per Ayurvedic Pharmacopeia of India (API). The samples of Neelibhrngadi Taila prepared were subjected to quality assessment by estimating acid value, refractive index and performing Thin Layer Chromatography (TLC) with absolute control as the standard, as per the recommendation of API, which defines well the quality check of the medicated oils. Least acid value (1.460mg of KOH g-1 of sample) was a desirable feature recorded in oil sample prepared using microwave blanched and vacuum dried leaf samples and the highest acid value recorded was in the sample prepared using hot water blanched cabinet dried leaves. There was no difference in the refractive index as coconut oil was the common base oil in all the treatments and it revealed the uniform extraction of phytochemicals in to the oil in all the 13 formulations. The results from TLC showed that all the oil samples except those obtained from treatment T10 (steam blanched and dried in vacuum drier) and T11 (Hot water blanched and dried in vacuum drier) gave standard Rf value, thus conforming to the quality prescribed by API. In HPTLC performed using absolute control as standard, all the samples recorded 13-17 peaks representing different phytochemical compounds whereas the standard oil recorded 16 peaks. Further studies are required to identify the phytochemical compounds.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810140408
dc.keywordsNeelamari, Indigo, Cutting stage and Storage time of neelamari leaves, Raw herbal drugs, Medicinal plantsen_US
dc.language.isoenen_US
dc.pages73en_US
dc.publisherDepartment of Processing Technology, College of Horticulture Vellanikkaraen_US
dc.subHorticultureen_US
dc.subjectnullen_US
dc.themePostharvest studiesen_US
dc.these.typeM.Scen_US
dc.titlePostharvest studies in neelamari (Indigofera tinctoria L.)en_US
dc.typeThesisen_US
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