Effect of heavy metals concentration on biomolecules and immunity parameters of earthworm, Eisenia fetida

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dc.contributor.advisorGupta, R.K.
dc.contributor.authorJatwani, Chhavi
dc.date.accessioned2017-06-03T04:30:29Z
dc.date.available2017-06-03T04:30:29Z
dc.date.issued2015
dc.description.abstractAmong terrestrial invertebrates, earthworms are priority test organisms for soil contamination surveys because they are easy to handle, widespread in their terrestrial distribution and have the capacity to accumulate and concentrate large quantities of inorganic and organic pollutants with acquired adaptive resistance to the toxicity of these pollutants including heavy metals. Biochemical responses in organisms against environmental stress are regarded as early warning indices of pollution in the environment. The present study was carried out to evaluate the effect of heavy metal on the biomolecules and immunity parameter of earthworm, Eisenia fetida. The results showed acute toxicity of two types of metals (mercury and cobalt) at different concentration on earthworms In the first experiment bio-molecules concentration in the heavy metal contaminated earthworm was estimated which shows the decrease in protein, carbohydrate and lipid concentration. The maximum reduction in bio-molecules i.e. 29.18% in protein 21.57% in carbohydrate and 41.25% in lipid concentration was seen in earthworms treated with Hg-0.06ppm. For other treatments i.e. Cobalt and combination of mercury and cobalt the results show decrease in bio-molecules but decrease was almost negligible. When the cells of coelomic fluid were calculated there was significant decrease in the total number of coelomocytes 82.54% in earthworms treated with Hg-0.06ppm similarly significant reduction in eleocytes, amoebocytes I amoebocytes II, granulocytes I and granulocytes II was seen 74.65% decrease was seen for earthworm treated with Co-0.06ppm and reduction was 79.73% for earthworms treated with combination of both i.e. Hg-0.03 + Co-0.03 ppm. The antioxidant activity was measured by DPPH scavenging activity by using two solvents i.e 75% methanol, 80% methanol and 80 % ethanol. 75% methanol and 80% ethanol was concluded as best solvent showing 47% inhibition activity whereas for 80% methanol it shows 38 % inhibition activity. Antioxidant activity for different heavy metal treated earthworm was studied w.r.t. control earthworms which show there is sharp increase in antioxidant activity in first 15 days and then become almost stable. Maximum increase in antioxidant activity at day 60 was seen for earthworms treated with Hg-0.06 ppm i.e. 60.718% which was 47.232% by using 80% ethanol as solvent. It was concluded that among two heavy metals, mercury is more toxic than cobalt and combination of both.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810014900
dc.language.isoenen_US
dc.publisherCCSHAUen_US
dc.subZoology
dc.subjectLivestock, Biological interaction, Surface water, Fruits, Animal husbandry, Irrigation, Suckering, Byproducts, Biological phenomena, Beveragesen_US
dc.these.typePh.D
dc.titleEffect of heavy metals concentration on biomolecules and immunity parameters of earthworm, Eisenia fetidaen_US
dc.typeThesisen_US
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