A Study On Vitreous Humor Between-Eye Differences And Baseline Values Of Potassium, Calcium, Sodium And Glucose Immediately After Death In Dogs
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Date
2014-09
Journal Title
Journal ISSN
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Publisher
TANUVAS, Chennai
Abstract
The present study was conducted to study the vitreous humor betweeneye
differences and baseline values of potassium, sodium, calcium and glucose
immediately after death in dogs. Eight canine carcasses brought for necropsy
formed the material for collection of vitreous humour in the present study.
The information regarding time of death was gathered from dog owners,
clinicians and clinical records. Eyes were enucleated as soon as possible after
receiving carcass for postmortem. Sampling of vitreous humor was done using
16 gauge needle and 20 ml syringe. The needle was inserted at the lateral
angle of eyeball so that the tip of needle was placed at the center of the eyeball.
About 50 μL vitreous humour was gently aspirated from each eye and
transferred to eppendorf tubes. Sampling of vitreous humour was done within
1-4 hours after death. The aspirated vitreous humour samples were centrifuged
at 13,000 rpm for 15 min at 50C temperature and the supernatant was used for
analysis. The average vitreous humour concentrations of potassium, sodium,
calcium and glucose estimated within 1-4 hours after death were 8.18±0.31
mEq/L, 152.55±4.46 mEq/L, 9.52 ±0.20 mg/dL and 21.70±4.50 mg/dL
respectively. The results of this study indicated that there were no significant
differences between left and right eye for all of the vitreous biochemical
constituents that were studied in present study (estimated within 1-4 hours
after death).
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Keywords
Veterinary Science, Veterinary Pathology