AMBIENCE ASSOCIATED VARIATIONS IN THE SERUM BIOMARKERS OF OXIDATIVE STRESS IN BUFFALOES OF ARID TRACT 'kq
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Date
2012
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Rajasthan University of Veterinary and Animal Sciences, Bikaner - 334001
Abstract
The understanding of the role of oxidants and antioxidants in stressed animals is gaining importance to overcome oxidative stress, however, very little emphasis has been given on this aspect in Murrah buffaloes. Therefore the present investigation was carried out on four hundred and fifty apparently healthy adult females between 4 and 12 years of age to determine the ambience associated variations in the serum biomarkers of oxidative stress in Murrah buffaloes of arid tract. In each ambience (moderate, hot and cold), according to physiological states, buffaloes of group A included nonpregnant milch; pregnant milch and pregnant dry animals and buffaloes of group B included primipara and multipara animals. Biomarkers of oxidative stress included serum enzymes and antioxidants. Serum enzymes were catalase(CAT); monoamine oxidase (MAO); glutathione reductase (GR); oxidase; peroxidase;super oxide dismutase (SOD);xanthine oxidase (XO); and gamma glutamyl transferase (GGT) whereas antioxidants were vitamin E; vitamin C; vitamin A and Beta
carotene and glutathione (GSH). Other serum analytes of the study were enzymes, metabolites and electrolytes. Serum enzymes were sorbitol dehydrogenase (SDH); malate dehydrogenase (MDH); glucose–6-phosphatase (G-6-Pase); glucose6- phosphate dehydrogenase(G-6-PD); 5’nucleotidase (5’NT); alkaline phosphatase (ALP); and ornithine carbamoyl transferase (OCT). Serum metabolites were urea; creatinine; total proteins; cholesterol; triglycerides and and glucose. Serum electrolytes were inorganic phosphorus, calcium and magnesium. The overall mean values of above mentioned parameters were 73.69±1.19 kU L-1; 289.25±5.20U L-1; 12.64±0.20kU L-1; 80.17 ± 2.55 mU L-1; 63.01±1.60 kU L-1; 284.18±2.75 mU L-1; 72.37±1.15 U L-1; 82.83±2.00U L-1; 3.54 ± 0.002; 19.78±0.34; 1.89±0.02; 2.20±0.03; 4.84±0.02; 10.00±0.04 ; 60.00±0.47 ; 11.32±0.10 ; 7.33±0.06; 6.00±0.05; 95.00±2.00 ; 60.33±1.02; 6.04±0.03 mmol L-1; 181.00±1.2 µmol L-1; 66.50±0.20 gL-1; 3.56±0.02 m mol L-1; 0.87±0.02 m mol L-1; and 3.53±0.02mmol L-1 ; 1.57±0.010 ; 2.42±0.01 ; and 0.93±0.002 , respectively, which were obtained from 450 animals irrespective of ambiences, sex and age. The mean values of serum MAO, GR, oxidase, peroxidase, SOD and GGT were significantly (p≤0.05) higher during hot and cold ambiences; of serum CAT and XO were significantly(p≤0.05) higher during hot ambience and significantly (p≤0.05) lower during cold ambience; of serum vitamin E, vitamin C, vitamin A, β carotene and glutathione were significantly (p≤0.05) lower during hot and cold ambiences; of serum SDH, MDH, G-6- Pase, 5’NT and ALP were significantly (p≤0.05) higher during hot and cold ambiences; of serum G-6-PDH was significantly (p≤0.05) higher during cold ambience while significantly (p≤0.05) lower during hot ambience; of serum OCT was significantly (p≤0.05) higher during hot and significantly (p≤0.05) lower during cold ambiences; of serum urea and creatinine were significantly (p ≤0.05) higher during hot and cold ambiences; of total serum proteins was significantly (p≤ 0.05) lower during hot and cold ambiences; of serum cholesterol and glucose were significantly (p≤0.05) lower during hot and higher during cold ambience; of serum triglycerides was significantly (p≤0.05) lower during hot and cold ambience; and serum inorganic phosphorus, calcium and magnesium were significantly (p≤0.05) lower during hot and cold ambiences, in comparison to respective overall moderate mean value. In each ambience the effects of physiological states were significant (p≤0.05) for each parameter.The interactions between ambience X group A(non pregnant milch, pregnant milch and pregnant dry); and ambience X group B(primipara and multipara) and between group A X group B were highly significant (p≤0.01) for each parameter.
It was concluded that extreme ambiences produced the oxidative stress in the Murrah buffaloes of all the physiological states, which was reflected in the form of altered status of the biomarkers of oxidative stress and other analytes determined in the serum. These findings indicated towards the modulation of physiological mechanisms in response to free radicals. The study will help in realistic evaluation of the management practices including antioxidant supplementation and diagnosis of disease conditions. The data obtained can also serve as a baseline for future research in the field of veterinary clinical physiology.
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