A study on certain physico-biochemical attributes of local Pantja Buck semen
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Date
2014-05
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G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand)
Abstract
Pantja is the local breed of goat found in Tarai region of Uttarakhand. In present investigation 6 local Pantja bucks B6, B5, B4, B3, B2 and B1 of age 11, 14, 15, 18, 20 and 34 months with body weights 16, 17, 21, 26, 28 and 40 kg respectively, maintained at Goat unit, LPM Department, College of Veterinary and Animal Sciences, Pantnagar, were selected to study certain physic-microscopic tests, complementary biochemical tests, biochemical attributes and elemental attributes of semen and morphology of sperm. Semen collection was done at about 9.009.30 a.m. by artificial vagina method twice weekly per buck. Immediately after collection, physical examination of fresh semen was done first and then microscopic examination of undiluted and diluted semen was performed. After that complementary biochemical tests were performed on fresh semen. For tests performed in seminal plasma, semen was centrifuged at 3000 rpm for 10 minutes and was stored at -18 ºC except for the tests performed in fresh seminal plasma. Sperm dimensions and abnormalities were also measured by microscopy. The results of the experiment indicated that maximum samples 58.33 and 33.66 per cent were creamy in color and creamy consistency and minimum 9.1 and 4.5 per cent were yellow in color and watery in consistency respectively. It was found that maximum samples obtained from older bucks were creamy in color and consistency. The volume of the semen increased with age of the bucks with an overall value of 0.54 ± 0.02 ml (range 0.38 ± 0.03 to 0.78 ± 0.04 ml). Mass motility, individual motility and sperm concentration of semen was increased with age of the animal and found maximum for buck B1. Mean sperm dimensions viz. sperm head length, head width, flagellum length and sperm total length by light microscope (100X) were found as 8.03 ± 0.02, 3.98 ± 0.02, 51.92 ± 0.11 and 60.05 ± 0.10 µm respectively. Average mean values of sperm head length, head width, head base width, head thickness, acrosome thickness, mid piece length, principal piece length, end piece length, mid piece thickness, principal piece thickness and total sperm length by SEM was recorded as 8.46 ± 0.03, 4.05 ± 0.02, 2.17 ± 0.01, 0.45 ± 0.00, 0.57 ± 0.00, 10.72 ± 0.61, 37.47 ± 0.07, 3.52 ± 0.66, 0.76 ± 0.02, 0.52 ± 0.00 and 60.17 ± 0.02 µm respectively. These parameters were independent of buck age. Average mean values of primary and secondary sperm abnormalities were found as 1.77 and 2.44 per cent respectively with overall total sperm abnormalities 4.22 per cent. Overall mean value of pH was found (6.61 ± 0.02) independent of buck age. Resazurin and Methylene blue reduction time were noted higher for younger bucks and minimum for older bucks with overall value 3.15 ± 0.25 and 6.04±0.36 min. respectively. Mean values of HOST and Millovanov’s resistance test were recorded as 36.10 ± 1.17 per cent and 5216.70 ± 1.94 respectively. The mean values of mass motility, individual motility and livability of sperms were found as 1.60 ± 0.09, 36.76 ± 0.69 per cent, 16.76 ± 0.86 per cent at 0°C, 1.93 ± 0.12, 47.36 ± 0.94 per cent, 27.30 ± 0.80 per cent at 10°C and 3.46 ± 0.37, 74.03 ± 0.82 per cent, 71.03±0.867 per cent at 37°C respectively. Overall mean values of glucose, total protein, albumin, globulin, A/G ratio, creatinine, urea, uric acid, fructose and cholesterol were found as 38.86 ± 0.78, 4.61 ± 0.11, 2.22 ± 0.05, 2.32 ± 0.06, 1.09 ± 0.04, 1.99 ± 0.03, 42.26 ± 0.99, 7.16 ± 0.14, 605.84 ± 14.26 and 152.10 ± 3.14 respectively. Concentrations of elements Mg, P, Cl, Zn, Cu, Fe and Ca in seminal plasma of Pantja bucks were 2.84 ± 0.05, 8.09 ± 0.24, 86.00 ± 4.94, 0.29 ± 0.06, 0.04 ± 0.00, 0.16 ± 0.03 and 10.50 ± 0.33 respectively. Concentration of most of biochemical and elemental constituents were found higher in mature animals as compared to yearlings. Results of present study reveal that Pantja buck produce good quality semen, having semen characteristics similar to other goat breeds of the country. They are therefore well suited for breeding purpose and can be used for natural services as well as production of semen for artificial insemination.
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