LINE X TESTER ANALYSIS IN POPULUS DELTOIDES BARTR.
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Date
2016
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Abstract
ABSTRACT The present investigation entitled “Line X Tester analysis in Populus deltoides Bartr” was carried out in the Department of Tree Improvement and Genetic Resources, Dr Y. S. Parmar University of Horticulture & Forestry Nauni, Solan (HP) during 2013-2015. There was a collection of Populus deltoides female and male clones/strains/species procured from State Forest Department, Haldwani and Shyampur, Haridwar Forest Division and maintained in the germplasm block of Naganji nursery from which 11 male and 13 female clones were included in the reproductive study and the selected plant material of 4 females (G-48, S1, S7C8 and L62/84) and 4 males (S 7 C 11 , L-17/92, L-124/86 and S 7 C 1 ) clones were crossed using Line x Tester (4 x 4) mating design. Control crossing was done and seedlings were raised in the nursery at stage-1 and were evaluated for morphological characters. In stage-2 clonal cuttings of selected individuals were raised in RBD and were evaluated for morphological, physiological and wood characters. Line x tester analysis for combining ability revealed that that line S1 and tester L-17/92 were found to be good general combiners. On the basis of heterosis over better control, mean performance and significant desirable SCA effects were recorded for morphological, physiological and wood characters and the combinations L-62/84 X S7C1 and L-62/84 X L-17/92 were found to be the most promising families. The estimates of SCA variance (σ2 SCA)) were more than the GCA variance (σ2 GCA for all the characters studied. The gene action study revealed that dominance variance was observed more than the additive variance for all the parameters studied. The proportional contribution of lines was higher than individual contribution of testers or line × tester interaction except for plant height, collar diameter, internodal length, leaf area, maximum width of leaf, angle between the mid rib and 2nd lower lateral mid rib, shoot bark thickness, root bark thickness, fresh shoot weight, dry shoot weight, dry root weight, dry root shoot ratio, root length, total fresh weight, total dry weight, stomatal conductance and fibre length. Among 18 SSR markers, fifteen markers showed monomorphic allelic pattern, the remaining three markers (PMGC-2060, PMGC-2020 and PMGC-451) showed polymorphic pattern
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Populus deltoides Bartr,Line X Tester analysis