DIFFERENTIAL METHODS FOR ANALYZING THE OUTER MEMBRANE PROTEINS OF PATHOGENIC SEROGROUPS OF LEPTOSPIRA
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Date
2015
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Abstract
The leptospiral outer membrane has a relatively complex protein profile
Pathogenic mechanisms of Leptospira interrogans, the causal agent of leptospirosis, remain
largely unknown. The outer membrane of pathogenic Leptospira species grown in culture
media contains lipopolysaccharide (LPS), a porin (OMPL), and several lipoproteins,
including LipL36 and LipL41. Loa22 was shown to be a protein having a C-terminal OmpA
consensus domain. Loa22 was detected among pathogenic leptospires but not among nonpathogenic
leptospires, suggesting the possible involvement of this protein in virulence.
Loa22 is located in the outer membrane and a small portionis exposed on the cell surface.
Thus, Loa22 may be a candidate for a novel vaccine against infection with pathogenic
leptospires. The purpose of the study was to analyze the outer membrane proteins of
pathogenic serogroups of leptospira by different methods whole cell solubilization, insoluble
method and detergent method to identify the best method for extraction of proteins. Results
of this study suggest that whole cell solubilization method was found promising for outer
membrane protein extraction.
Description
TNV_IJSET_2015_4(1)101-107
Keywords
Veterinary Science