ASSESSMENT OF GENETIC DIVERSITY IN NAG CHHATRI (Trillium govanianum Wall. ex D.Don) – AN IMPORTANT MEDICINAL HERB OF HIMALAYAN REGION
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Date
2021-11
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UHF,NAUNI
Abstract
ABSTRACT
The present study was aimed to assess genetic diversity based on PCR-based
molecular markers in an endangered ethno medicinal herb of Himalayan region, Nag chhatri
(Trillium govanianum Wall.ex D.Don). Total 38 genotypes were procured from high altitude
regions comprising of four districts viz., Kullu, Shimla, Chamba and Sirmaur of Himachal
Pradesh and were characterized based on ISSR and SSR marker analyses. Based on obtained
DNA profiles as well as factorial and neighbour joining tree analyses, minimal genetic
diversity was observed using both the markers. On an average, the genotypes from Kullu and
Chamba regions depicted to be more diversified compared to each other with respect to the
other two locations taken into consideration. The admixture of two genetic pools (K=2) was
observed among the subjected genotypes using structure programme version 2.3.4. The low
levels of genetic diversity could be due to the immense population decrease, vegetative
proliferation, presence of meiotic irregularities towards pollen sterility, poor seed viability,
inbreeding depression, limited seed dispersal, slow life cycle and specific habitat
requirement, etc. Therefore, the present findings are emphasizing on executing efficient
prioritized effective means (ex-situ propagation measures and strict in-situ conservation
policies) towards its biodiversity conservation due to limited gene flow among the
populations in natural habitat