Genic status in relation to radlosensltlvity, mutation frequency and spectrum in Bhindi

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Date
1985
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Department of Agricultural Botany, College of Agriculture, Vellayani
Abstract
The effect of 30 RR ^Co-gamma rays on different genotypes of Bhindi (Abelmoschus esculentus Moench) have been studied in detail in M1 and M2 generations, The experiments were conducted during 1981-83 at the Department of Agricultural Botany, College of Agriculture, Vallayani, There were 20 different genotypes including both pure and hybrid seeds. The M1 generation was laid out in RBD with two replications and maintained following the package of practices recommended for this particular crop. The radio sensitivity of the different genotypes wore tested based on the direct effect of the mutagen in M1 generation. The treated hybrid materials along with their control, parental varieties and 30 kR exposed parental types were carried further to H2 generation to assess the extent of variation created in the segregating M2 generation. Proper lay out, maintenance of the crop and data collection were followed in segregating generation also. The data collected were statistically analysed for proper interpretation of the results obtained. To assess the direct effect of the mutagen in M1 generation various growth metrics such as germination percentage, days to complete germination, plant height, number of loaves and branches per plant at 30 days interval, pollen and seed sterility and various fruit characters including yield per plant were taken into consideration. All the characters analysed showed difference in expression depending on the genotypes concerned. Majority of the growth characters showed significant reduction in gaiapa ray treated population compared to their respective controls. A delay in germination was noted in majority of the genotypes. Growth metric analysis clearly demonstrated that eventhough treatment delays the crop growth in early stages, at later phases of growth the plant rectifies itself and attains maximum expression as in the case of control population. All the genotypes tested showed increased pollen and seed sterility due to gamma ray exposure, which directly influences the number of fruits produced per plant. Yield per plant showed significant variation among the treatments and in majority of the genotypes gamma rays significantly reduced yield potentiality of the plants* Based on seed sterility percentage, it was possible to group the plants under low, medium and high sterile types.
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