Occurrence of SHV GENE and Antibiogram of Klebsiella Pneumoniae Infection in Swiss Albino Mice Colony
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Date
2016
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Abstract
An epizootic causing high morbidity and high mortality in Swiss albino inbred mice over
a period of two weeks caused by Klebsiella pneumoniae is reported. Diagnosis was based
on clinical signs, histopathological, bacteriological evaluation of the lung, kidney and
intestinal tissue and multiplex PCR. Biochemical tests were positive for citrate, nitrate,
urease, lysine decarboxylase, ornithine decarboxylase and negative for phenylalanine
and hydrogen sulphide production. Carbohydrates viz., glucose, lactose, adonitol,
arabinose were fermented but sorbitol was not. Disc diffusion tests for antibiotic
sensitivity showed susceptibility to enrofloxacin, amikacin, doxycyline and resistant to
cephalexin, cefadroxil, cefuroxime, cephtriaxone and ampicillin. Amplification by
multiplex PCR for sulphydryl variable (SHV) gene yielded a product size of 472bp.
Description
TNV_IJAS_2016_3(3)479-483
Keywords
Veterinary Science