MOLECULAR VARIABILITY OF FUSARIUM UDUM BUTLER.
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Date
2019-08-05
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Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, Maharashtra.
Abstract
The present investigation entitled, “Molecular Variability of Fusarium udum Butler” was carried out at Department of Plant Pathology, Dr. PDKV, Akola. Rapid roving survey was carried out during Kharif 2014-15 and 2015-16 season in major pigeonpea growing areas of India and revealed 8.94 to 48.33 per cent variation for wilt incidence. Wilt incidence was observed throughout the all growth stages of pigeonpea plants but more severe at vegetative, flowering and poding stage.Twenty one isolates of F. udum selected for morphological variability studies belong to different agro-climatic zones. All F. udum isolates showed variation in morphology like, colony growth pattern, colony colour, radial growth rates and pigmentation.
Screening of F. udum isolates by pot culture method on susceptible variety (ICP-2376) observed high degree of variation for wilt incidence (40–95%) and average expression of wilting was observed from 37 to 45 DAS. Moderately resistant variety (PKV Tara) showed variation for wilt incidence (0–65%) and average expression of wilting was observed from 44 to 58 DAS. However, resistant variety (Asha) showed variation for wilt incidence (0-55%) and average expression of wilting was observed from 55 to 65 DAS. Screening of F. udum isolates by water culture method on susceptible variety (ICP-2376) found high degree of variation for wilt incidence (33.33–100%) and average expression of wilting was observed from 5 to 10 DAI. Moderately resistant variety (PKV Tara) showed variation for wilt incidence (0–100%) and Average expression of wilting was observed from 0 to 15 DAI. However, resistant variety (Asha) exhibited variation for wilt incidence (0-50%) and average expression of wilting was observed from 0 to 22 DAI.Screening of F. udum isolates by culture filtrate method on susceptible variety ICP-2376 were recorded degree of pathogenic variability first appearance of disease symptoms after inoculation, and it was found to vary from 1 to 4 days and moderately resistance variety PKV Tara were showed variability first appearance of disease symptoms after inoculation, and it was found to vary from 1 to 6 days.
The genetic diversity of 21 isolates of F. udum was studied using three molecular markers (SSR, ISSR and RAPD). Among 9 SSR primers screened, 8 primers showed good amplification and generated 39 amplicons. The per cent polymorphism generated by SSR primers were 100 per cent among all 21 isolates of F. udum. The genetic similarity coefficient value ranged from 0.44 to 0.85 across twenty one isolates. Dendrogram generated using SSR amplicons grouped all the 21 isolates into six major clusters at 72 per cent similarity coefficient.The molecular variability by ISSR primers showed total 49 scorable and reproducible amplicons with an average 4.9 amplicon per primer. The per cent polymorphism generated by ISSR primers were 93.88 per cent among all 21 isolates of F. udum. The genetic similarity coefficient value ranged from 0.41 to 0.92 across twenty one isolates of F. udum indicating high degree of variation in respect to genetic similarity. The dendrogram generated by SHAN clustering programme using UPGMA grouped all the isolates into seven major clusters at 72 per cent similarity coefficient. Variability by RAPD primers were produced total 176 scorable and reproducible amplicons with an average 8.80 amplicon per primer. The per cent polymorphism generated by RAPD primers were 83.52 per cent among all 21 isolates of F. udum. The genetic similarity coefficient value ranged from 0.57 to 0.90 across twenty one isolates of F. udum indicating high degree of variation in respect to genetic similarity. The dendrogram constructed by UPGMA from the pooled data of RAPD grouped all the isolates into eight major clusters at 76 per cent similarity coefficient.
Description
Experiment was conducted on molecular variability of Fusarium udum Butler at Department of Plant Pathology, Dr. P. D. K. V., Akola. Rapid roving survey was carried out during Kharif 2014-15 and 2015-16 season, collection of wilt sample and pathogenicity proved by pot and water culture method and also molecular variability was carried out by using molecular marker (SSR, ISSR and RAPD). The data were analysed by Numerical Taxonomy System Version 2.2.
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Citation
VYAVHARE, GANESH FAKIRA. (2019). Molecular variability of Fusarium udum Butler. Department of plant pathology, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola. Ph. D. 2019. Print. xv, 158p. (Unpublished).