STUDY ON HISTOGENESIS OF KIDNEY IN SHEEP (Ovis aries)
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Date
2017-08
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SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA
Abstract
ABSTRACT:
The study was carried out in 45 embryos and foetuses of local Nellore sheep
from 20 days to 150 days of gestation to explicate the development of various
structures in the kidney. At 20 days (0.7cm CRL) of embryo, the mesonephros located lateral to the testis and occupied larger part of the coelomic cavity. In group I (39.5 days) to group III (150 days) the dorsal surface of both the metanephric kidneys was in contact with psoas major muscle. The metanphric kidneys, in all age groups were varying in colour from light to dark brown. In 20 day (0.7cm CRL) old sheep embryo the pronephros was placed on either side of the dorsal aorta which was surrounded by undifferentiated mesenchymal cells. The pronephros consisted of only one giant glomerulus of 225±1.3μm in size. At 24 days (1.1cm CRL) degenerative changes were noticed in glomerular network due to which vacuolation was noticed between the tuft of glomerulus. In 20 day (0.7cm CRL) old embryos the mesonephric parenchyma was densely packed with tubules, migrating angioblasts and differentiating glomerulus. At 24 days (1.1cm CRL) the mesonephric glomerulus
surrounding visceral and parietal layers were demarcated at this age. In 27 days
(1.7cm CRL) the manifestation of macula densa of DCT, Juxtaglomerular cells of
afferent arteriole and mesengial cells at vascular pole of Bowman’s capsule was
observed. At 49 days (7cm CRL) of gestation glomerulus shrinkage was noticed
indicating the degeneration process initiated in mesonephros. The ureteric bud of
metanephros was formed by evagination of the caudal part of the mesonephric duct in sheep embryo at 20 days of gestation (0.7cm CRL). At 44 days (5cm CRL) of
gestation the number of the renal corpuscles were increased and distributed towards
periphery from the pelvis. Demarcation between proximal and distal tubules was
noticed. Major and minor calyces were apparent at 57 to 60 days of gestation and
increased quantity of collagen at the sinus around the major and minor calyces. Loop
of Henle was first appeared at 83.5 days, which were well established in juxta
medullary nephrons as descending and ascending limbs. In group III the typical cortex and medulla was differentiated as adult kidney and renal corpuscles were still under differentiating stages in the cortical region by 101 days. Papillary ducts were lined by stratified cuboidal epithelium minor calyx was lined by transitional epithelium like adult kidney and smooth muscle were also noticed in the wall of the calyx minor in 115 days of gestation. By 120 days Juxta glomerular cells exhibited the cytoplasmic granules. In group III at 101 days (26cm CRL) the capsule and tubules showed mild PAS positive reaction and the cytoplasm of collecting ducts showed intense PAS positive indicating the neutral mucopolysccharides in the collecting ducts. In group I to group III the renal corpuscles, tubules and collecting ducts showed negative reaction for both acid and alkaline phosphatases
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