Evaluation and molecular characterization of vegetable type pigeonpea [Cajanus cajan (L)] genotype for nutritional quality and yield attributing traits.
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Date
2017-06-30
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Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola
Abstract
The present investigation entitled “Evaluation and molecular characterization of vegetable type pigeonpea [Cajanus cajan (L)] genotype for nutritional quality and yield attributing traits” was conducted in the experimental field of Department of Agricultural Botany, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, Maharashtra state during Kharif of 2016-2017 to elucidate the information about evaluation and identification of desirable vegetable type pigeon pea [Cajanus cajan (L.)] genotype for nutritional quality and yield attributing traits as well as to assessing genetic analysis for morpho-physiological, nutritional quality and yield attributing traits. The study also depicted the information about molecular characterization of promising vegetable type pigeon pea genotypes.
Six nutritional traits viz. carbohydrate, protein, calcium, iron, manganese, and zinc revealed that the green stage legumes had higher amounts of minerals viz., iron and zinc. However the grain stage legumes had higher amount of protein, starch manganese and calcium. The Principal component analysis (PCA) revealed that 71.50% of the total diversity was explained on the basis of the first four principal components. The first principal component (PC1) had the highest Eigen value of 3.649 and explained 26.064% of total variation. Whereas, the fourth principal component (PC4) accounted for only 9.827% of the variation with an Eigen value of 1.376, which is the lowest value among the four principal components. The cluster diagram which was done to evaluate the sixteen genotypes based on nutritional data, yield attributing characters and morrpho-physiological traits respectively revealed that overall four clusters were formed in cluster diagram based on nutritional data; five clusters were generated for yield attributing characters whereas only three clusters were generated for morpho-physiological traits, which showed a wide range of variability between the genotypes under study.
The second objective of the study was genetic analysis for morpho-physiological, nutritional quality and yield attributing traits. The field component of the experiment included sixteen genotypes of pigeonpea that spread over season, Kharif,2016-17. The crop was grown in three replications using two lines for each of the genotype.
The observations recorded were days to 50 % flowering, fresh pod colour, flower petal colour, plant height, leaf area index both (R6 and R8), chlorophyll content index both (R6 and R8), Days to harvest at first flush for vegetable purpose (R6), number of primary branches per plant, 100 fresh pod weight, 100 fresh seed weight, pod length, pod width and green pod yield per plant.
A wide range of variability was observed for all the characters. The estimates of phenotypic coefficients of variation were higher than that of genotypic coefficients of variation for all characters under study.
The estimates of genotypic correlation coefficient were higher than phenotypic correlation coefficient, indicating little masking effect environment on the expression of the characters.
The best genotype for green pod yield was found BDN-716. High heritability accompanied with high genetic advance was observed for the trait 100 fresh pod weights. This trait can be used for selection in the further breeding programmes.
Molecular characterization of promising vegetable type pigeon pea genotypes showed highest similarity (0.762) between AMAR and AKTM-11-06, whereas the lowest similarity coefficient was observed between SKN-0632 and AKTM-11-06 with a similarity coefficient value of (0.056). UPGMA dendrogram constructed using Jaccard’s similarity coefficient gave the clustering of pigeonpea genotypes. The genotypes were clustered into two super clusters viz. super cluster-I and super cluster-II. The cluster analysis revealed that high diversity was observed in PKV-TARA and AKTM-1644. These diverse genotypes may be used as valuable sources for breeding programmes.
Description
In the present investigation entitled “Evaluation and molecular characterization of vegetable type pigeonpea [Cajanus cajan (L)] genotype for nutritional quality and yield attributing traits” was conducted in the experimental field of Department of Agricultural Botany, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, Maharashtra state during Kharif of 2016-17. Moreover, such experiment help to elucidate the information about evaluation and identification of desirable vegetable type pigeon pea [Cajanus cajan (L.)] genotype for nutritional quality and yield attributing traits as well as to assessing genetic analysis for morpho-physiological, nutritional quality and yield attributing traits. The study also depicted the information about molecular characterization of promising vegetable type pigeon pea genotypes. The study was also elucidate the genetic analysis for morpho-physiological, nutritional quality and yield attributing traits. The molecular variability study was also done from which it was clear that the variation was not only in genotype level but also in DNA level.
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Citation
SINGH, SANDEEP KUMAR. (2017). Evaluation and molecular characterization of vegetable type pigeonpea [cajanus cajan (l)] genotype for nutritional quality and yield attributing traits, Department of Agricultural Botany (Genetics and Plant Breeding). Dr. Panjabrao Deshmukh Krishi Vidyapeeth, M.Sc.2017, print, xviii, 113p, (Unpublished)