NEUROPROTECTIVE EFFECT OF OVINE WHARTON’S JELLY DERIVED MESENCHYMAL STEM CELLS ON PERIPHERAL NERVE REGENERATION IN RAT MODELS
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Date
2022
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Abstract
The present study was designed to compare the in vivo neuroregenerative effect of ovine Wharton’s jelly derived MSCs (WJ-MSCs) and neuronally trans-differentiated ovine WJ-MSCs on periphery injury in rat model and to evaluate the neuronal trans-differentiation potential of ovine WJ-MSCs with appropriate characterisation. Efforts were also made to appraise the change in immunogenicity profile of ovine WJ-MSCs following neuronal differentiation
Ovine WJ-MSCs were isolated, cultured and characterized for their expression of MSC specific surface markers (CD73, CD44 and CD90), pluripotency markers (Oct4, Sox2 and Nanog), absence of expression of hematopoietic stem cell marker (CD34) and multi-lineage differentiation potential. Proliferation capabilities, population doubling timeV and clonogenicity assay was done to further define ovine WJ-MSCs.
The ovine WJ-MSCs were subjected to neuronal trans-differentiation with the aid of growth factors and chemicals and the differentiation was assessed by change in phenotype, nissl granule visualisation and level of expression of neuron specific markers like nestin, β III tubulin, glial fibrillary acidic protein (GFAP) and microtubule associated protein-2 (MAP2). The level of neurotrophins like nerve growth factor (NGF) and brain derived neurotrophic factor (BDNF) secreted in condition media of ovine WJ-MSCs and ovine neuronally differentiated WJ-MSCs were quantified by enzyme linked immunosorbent assay (ELISA) which showed significantly high level of BDNF in ovine neuronally differentiated WJ-MSCs compared to ovine WJ-MSCs, whereas level of NGF was significantly high in ovine WJ-MSCs in comparison to ovine differentiated WJ-MSCs.